Glycerol as substrate and NADP + -dependent glyceraldehyde-3-phosphate dehydrogenase enable higher production of 3-hydroxypropionic acid through the β-alanine pathway in E. coli.

Autor: Batista RS; Graduate Program of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luís, km 235, São Carlos, São Paulo 13565-905, Brazil., Chaves GL; Graduate Program of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luís, km 235, São Carlos, São Paulo 13565-905, Brazil., Oliveira DB; Graduate Program of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luís, km 235, São Carlos, São Paulo 13565-905, Brazil., Pantaleão VL; Department of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luís, km 235, São Carlos, São Paulo 13565-905, Brazil., Neves JDDS; Graduate Program of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luís, km 235, São Carlos, São Paulo 13565-905, Brazil., da Silva AJ; Graduate Program of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luís, km 235, São Carlos, São Paulo 13565-905, Brazil; Department of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luís, km 235, São Carlos, São Paulo 13565-905, Brazil. Electronic address: adilsonjs@ufscar.br.
Jazyk: angličtina
Zdroj: Bioresource technology [Bioresour Technol] 2024 Feb; Vol. 393, pp. 130142. Date of Electronic Publication: 2023 Dec 02.
DOI: 10.1016/j.biortech.2023.130142
Abstrakt: Microbial engineering is a promising way to produce3-HP using biorenewable substrates such as glycerol. However, theglycerol pathway to obtain 3-HPrequires vitamin B-12, which hinders its economic viability. The present work showed that 3-HP can be efficiently produced from glycerol through the β-alanine pathway. To develop a cell factory for this purpose, glycerol was evaluated as a substrate and showed more than two-fold improved 3-HP production compared to glucose. Next, the reducing power was modulated by overexpression of an NADP+ -dependent glyceraldehyde-3-phosphate dehydrogenase coupled with CRISPR-based repression of the endogenous gapA gene, resulting in a 91 % increase in 3-HP titer. Finally, the toxicity of 3-HP accumulation was addressed by overexpressing a putative exporter (YohJK). Fed-batch cultivation of the final strain yielded 72.2 g/L of 3-HP and a productivity of 1.64 g/L/h, which are the best results for the β-alanine pathway and are similar to those found for other pathways.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: