Impact of aminopenicillin administration routes on antimicrobial effects of platelet-rich fibrin: An in-vitro investigation.

Autor: Straub A; Department of Oral and Maxillofacial Plastic Surgery, University Hospital Würzburg, Pleicherwall 2, Würzburg 97070, Germany. Electronic address: Straub_A@ukw.de., Utz C; Department of Oral and Maxillofacial Plastic Surgery, University Hospital Würzburg, Pleicherwall 2, Würzburg 97070, Germany., Stapf M; Institute for Pharmacy and Food Chemistry, University of Würzburg, Am Hubland, Würzburg 97074, Germany., Vollmer A; Department of Oral and Maxillofacial Plastic Surgery, University Hospital Würzburg, Pleicherwall 2, Würzburg 97070, Germany., Breitenbuecher N; Department of Oral and Maxillofacial Plastic Surgery, University Hospital Würzburg, Pleicherwall 2, Würzburg 97070, Germany., Kübler AC; Department of Oral and Maxillofacial Plastic Surgery, University Hospital Würzburg, Pleicherwall 2, Würzburg 97070, Germany., Brands RC; Department of Oral and Maxillofacial Plastic Surgery, University Hospital Würzburg, Pleicherwall 2, Würzburg 97070, Germany., Hartmann S; Department of Oral and Maxillofacial Plastic Surgery, University Hospital Würzburg, Pleicherwall 2, Würzburg 97070, Germany., Lâm TT; Institute for Hygiene and Microbiology, University of Würzburg, Josef-Schneider-Straße 2/E1, Würzburg 97080, Germany.
Jazyk: angličtina
Zdroj: Journal of stomatology, oral and maxillofacial surgery [J Stomatol Oral Maxillofac Surg] 2024 Jun; Vol. 125 (3), pp. 101725. Date of Electronic Publication: 2023 Dec 02.
DOI: 10.1016/j.jormas.2023.101725
Abstrakt: Introduction: The aim of the study was to investigate the impact of different aminopenicillin administration routes on the antimicrobial effects of platelet-rich fibrin (PRF).
Methods: We enrolled patients undergoing treatment with amoxicillin/clavulanic acid (AMC) orally or ampicillin/sulbactam (SAM) intravenously. AMC was applied in a single oral dose (875/125 mg), or in a double oral dose (1750/250 mg), and SAM in a dose of 2000/1000 mg. Blood was obtained one hour after the intake of AMC or 15 min after the infusion of SAM ended. Antimicrobial effects were investigated in agar diffusion tests with fresh PRF, PRF stored for 24, and PRF stored for 48 h. Agar diffusion tests were performed with Escherichia coli, Staphylococcus aureus, Streptococcus pneumoniae, Haemophilus influenzae, and Porphyromonas gingivalis. Inhibition zones (IZs) around a 6 mm PRF disc were measured after 24 h.
Results: IZs for fresh PRF and the single oral dose of AMC were 0.0, 4.7, 15.2, 2.3, and 0.9 mm (E. coli, S. aureus, S. pneumoniae, H. influenzae, and P. gingivalis, respectively). For the double oral dose, these values were 0.0, 11.4, 20.0, 8.1, and 7.4 mm. IZs for SAM were 11.9, 18.2, 24.7, 20.3, and 22.1 mm. Differences between parenteral and oral application as well as between different oral doses were significant (p<0.0001, one-way ANOVA).
Discussion: The results of our study demonstrate that oral administration is a suitable route to load PRF with these drugs. This could expand the scope of PRF application to prevent infections at the surgical site, especially in an outpatient setting in which drugs are normally applied orally.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
Databáze: MEDLINE
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