IL-10 dependent modulatory effect of regulatory B10 cells on local scar formation following Roux-en-Y choledochojejunostomy in a novel rat model.

Autor: Ren ZY; Department of Hepaticbiliary Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, PR China., Wang J; Department of Thoracic Surgery, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, PR China., Xu F; School of Biomedicine, Beijing City University, Beijing 100083, PR China., Gao Y; Department of Thoracic Surgery, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, PR China., He Q; Department of Hepaticbiliary Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, PR China., Pan B; Department of Hepaticbiliary Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, PR China. Electronic address: panbingccmu@163.com., Lyu SC; Department of Hepaticbiliary Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, PR China. Electronic address: shaocheng0502@163.com.
Jazyk: angličtina
Zdroj: International immunopharmacology [Int Immunopharmacol] 2024 Jan 05; Vol. 126, pp. 111309. Date of Electronic Publication: 2023 Dec 04.
DOI: 10.1016/j.intimp.2023.111309
Abstrakt: Choledochojejunostomy has been common surgical treatment of biliary tract disease. Scar formation at anastomotic often results in postoperative complications associated with bleak post-operative recovery, in which local inflammation may be a potential target to modulate local scar formation. This study investigated the effect of regulatory B10 cells on local scar formation through interleukin-10 signal pathway following Roux-en-Y choledochojejunostomy (RCJS) in a novel rat model. Sprague-Dawley (SD) rats with RCJS were randomly divided into blank group, experimental group, IL-10 blocking group, control group, and received different interventions and duration. Injected through dorsal vein of penis, rats in different groups were treated respectively according to scheme. These interventions were performed during surgery, on 1st day, and 2nd day after surgery. Related indexes, including blood examination, specimen tissue of anastomotic detection, were recorded and compared in different interventional groups. Rats in experimental groups had more rapid recovery in liver function and inflammatory index, and higher in IL-10 level. Flow cytometry analysis showed that rats in experimental groups had highest content of B10 cells and lowest content of CD4 + CD25 - T cells in peripheral blood. Wider anastomotic by macroscopical observation, and slighter proliferation of collagen fiber and smooth muscle fiber, lower α-SMA and TGF-β1 levels by pathological staining were detected in experimental groups. Higher expression of the IL-10 gene and lower expression of TGF-β1 at anastomotic were detected in experimental groups. B10 cells may relieve local inflammation of anastomotic following RCJS in rats through IL-10-dependent modulatory effect, and improve local scar formation.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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