Normal sperm head morphometric reference values in fertile Asian males.

Autor:	Jia YL; Laboratory of Andrology, West China Second University Hospital Human Sperm Bank, Chengdu 610000, China., Wu YB; Laboratory of Andrology, West China Second University Hospital Human Sperm Bank, Chengdu 610000, China., Yu L; Laboratory of Andrology, West China Second University Hospital Human Sperm Bank, Chengdu 610000, China., Zheng Y; Laboratory of Andrology, West China Second University Hospital Human Sperm Bank, Chengdu 610000, China., Yang TT; Laboratory of Andrology, West China Second University Hospital Human Sperm Bank, Chengdu 610000, China., Wang YY; Laboratory of Molecular Translational Medicine, Center for Translational Medicine, Chengdu 610061, China., Zhou B; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu 610061, China., Zhang L; NHC Key Laboratory of Chronobiology, West China Second University Hospital, Sichuan University, Chengdu 610000, China., Li FP; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu 610061, China.
Jazyk:	angličtina
Zdroj:	Asian journal of andrology [Asian J Androl] 2024 May 01; Vol. 26 (3), pp. 315-320. Date of Electronic Publication: 2023 Dec 01.
DOI:	10.4103/aja202356
Abstrakt:	Sperm head morphology is crucial for male factor infertility diagnosis and assessment of male reproductive potential. Several criteria are available to analyze sperm head morphology, but they are limited by poor methodology comparability and population applicability. This study aimed to explore comprehensive and new normal morphometric reference values for spermatozoa heads in fertile Asian males. An automated sperm morphology analysis system captured 23 152 stained spermatozoa from confirmed fertile males. Of these samples, 1856 sperm head images were annotated by three experienced laboratory technicians as "normal". We employed 14 novel morphometric features to describe sperm head size (head length, head width, length/width ratio, and girth), shape (ellipse intersection over union, girth intersection over union, short-axis symmetry, and long-axis symmetry), area (head, acrosome, postacrosomal areas, and acrosome area ratio), and degrees of acrosome and nuclear uniformity. This straight-forward method for the morphometric analysis of sperm by accurate visual measurements is clinically applicable. The measured parameters present valuable information to establish morphometric reference intervals for normal sperm heads in fertile Asian males. The presented detailed measurement data will be valuable for interlaboratory comparisons and technician training. In vitro fertilization and andrology laboratory technicians can use these parameters to perform objective morphology evaluation when assessing male fertilization potential. (Copyright © 2023 Copyright: © The Author(s)(2023).)
Databáze:	MEDLINE
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