Targeted amplification-based whole genome sequencing of Monkeypox virus in clinical specimens.

Autor:	Isabel S; Public Health Ontario Laboratory, Public Health Ontario , Toronto, Ontario, Canada., Eshaghi A; Public Health Ontario Laboratory, Public Health Ontario , Toronto, Ontario, Canada., Duvvuri VR; Public Health Ontario Laboratory, Public Health Ontario , Toronto, Ontario, Canada.; Department of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto , Toronto, Ontario, Canada., Gubbay JB; Public Health Ontario Laboratory, Public Health Ontario , Toronto, Ontario, Canada.; Department of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto , Toronto, Ontario, Canada., Cronin K; Public Health Ontario Laboratory, Public Health Ontario , Toronto, Ontario, Canada., Li A; Public Health Ontario Laboratory, Public Health Ontario , Toronto, Ontario, Canada., Hasso M; Public Health Ontario Laboratory, Public Health Ontario , Toronto, Ontario, Canada., Clark ST; Public Health Ontario Laboratory, Public Health Ontario , Toronto, Ontario, Canada., Hopkins JP; Public Health Ontario Laboratory, Public Health Ontario , Toronto, Ontario, Canada.; Department of Health Research Methods, Evidence, and Impact, McMaster University , Hamilton, Ontario, Canada., Patel SN; Public Health Ontario Laboratory, Public Health Ontario , Toronto, Ontario, Canada.; Department of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto , Toronto, Ontario, Canada., Braukmann TWA; Public Health Ontario Laboratory, Public Health Ontario , Toronto, Ontario, Canada.
Jazyk:	angličtina
Zdroj:	Microbiology spectrum [Microbiol Spectr] 2024 Jan 11; Vol. 12 (1), pp. e0297923. Date of Electronic Publication: 2023 Dec 04.
DOI:	10.1128/spectrum.02979-23
Abstrakt:	Importance: We present a protocol to efficiently sequence genomes of the MPXV-causing mpox. This enables researchers and public health agencies to acquire high-quality genomic data using a rapid and cost-effective approach. Genomic data can be used to conduct surveillance and investigate mpox outbreaks. We present 91 mpox genomes that show the diversity of the 2022 mpox outbreak in Ontario, Canada. Competing Interests: J.B.G. was a paid consultant scientific editor for GIDEON Informatics, Inc., which was unrelated to the current work.
Databáze:	MEDLINE
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