Characterization of the Entner-Doudoroff pathway in Pseudomonas aeruginosa catheter-associated urinary tract infections.

Autor:	El Husseini N; Cell Biology and Molecular Genetics, University of Maryland at College Park , College Park, Maryland, USA., Mekonnen SA; Cell Biology and Molecular Genetics, University of Maryland at College Park , College Park, Maryland, USA., Hall CL; Cell Biology and Molecular Genetics, University of Maryland at College Park , College Park, Maryland, USA., Cole SJ; Cell Biology and Molecular Genetics, University of Maryland at College Park , College Park, Maryland, USA., Carter JA; Cell Biology and Molecular Genetics, University of Maryland at College Park , College Park, Maryland, USA., Belew AT; Cell Biology and Molecular Genetics, University of Maryland at College Park , College Park, Maryland, USA., El-Sayed NM; Cell Biology and Molecular Genetics, University of Maryland at College Park , College Park, Maryland, USA.; Center for Bioinformatics and Computational Biology, University of Maryland at College Park , College Park, Maryland, USA., Lee VT; Cell Biology and Molecular Genetics, University of Maryland at College Park , College Park, Maryland, USA.
Jazyk:	angličtina
Zdroj:	Journal of bacteriology [J Bacteriol] 2024 Jan 25; Vol. 206 (1), pp. e0036123. Date of Electronic Publication: 2023 Dec 04.
DOI:	10.1128/jb.00361-23
Abstrakt:	Pseudomonas aeruginosa is an opportunistic nosocomial pathogen responsible for a subset of catheter-associated urinary tract infections (CAUTI). In a murine model of P. aeruginosa CAUTI, we previously demonstrated that urea within urine suppresses quorum sensing and induces the Entner-Doudoroff (E-D) pathway. The E-D pathway consists of the genes zwf , pgl , edd , and eda . Zwf and Pgl convert glucose-6-phosphate into 6-phosphogluconate. Edd hydrolyzes 6-phosphogluconate to 2-keto-3-deoxy-6-phosphogluconate (KDPG). Finally, Eda cleaves KDPG to glyceraldehyde-3-phosphate and pyruvate, which enters the citric acid cycle. Here, we generated in-frame E-D mutants in the strain PA14 and assessed their growth phenotypes on chemically defined and complex media. These E-D mutants have a growth defect when grown on glucose or gluconate as the sole carbon source, which is similar to results previously reported for PAO1 mutants lacking E-D genes. RNA-sequencing following short exposure to urine revealed minimal gene regulation differences compared to the wild type. In a murine CAUTI model, virulence testing of E-D mutants revealed that two mutants lacking zwf and pgl showed minor fitness defects. Infection with the ∆ pgl strain exhibited a 20% increase in host survival, and the ∆ zwf strain displayed decreased colonization of the catheter and kidneys. Consequently, our findings suggest that the E-D pathway in P. aeruginosa is dispensable in this model of CAUTI. IMPORTANCE Prior studies have shown that the Entner-Doudoroff pathway is up-regulated when Pseudomonas aeruginosa is grown in urine. Pseudomonads use the Entner-Doudoroff (E-D) pathway to metabolize glucose instead of glycolysis, which led us to ask whether this pathway is required for urinary tract infection. Here, single-deletion mutants of each gene in the pathway were tested for growth on chemically defined media with single-carbon sources as well as complex media. The effect of each mutant on global gene expression in laboratory media and urine was characterized. The virulence of these mutants in a murine model of catheter-associated urinary tract infection revealed that these mutants had similar levels of colonization indicating that glucose is not the primary carbon source utilized in the urinary tract. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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