Immunogenicity and protective efficacy of recombinant adenovirus expressing a novel genotype G2b PEDV spike protein in protecting newborn piglets against PEDV.

Autor:	Song X; College of Animal & Veterinary Sciences, Southwest Minzu University , Chengdu, China., Zhou Q; College of Animal & Veterinary Sciences, Southwest Minzu University , Chengdu, China., Zhang J; College of Animal & Veterinary Sciences, Southwest Minzu University , Chengdu, China., Chen T; College of Animal & Veterinary Sciences, Southwest Minzu University , Chengdu, China., Deng G; College of Animal & Veterinary Sciences, Southwest Minzu University , Chengdu, China., Yue H; College of Animal & Veterinary Sciences, Southwest Minzu University , Chengdu, China.; Key Laboratory of Ministry of Education and Sichuan Province for Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization , Chengdu, China., Tang C; College of Animal & Veterinary Sciences, Southwest Minzu University , Chengdu, China.; Key Laboratory of Ministry of Education and Sichuan Province for Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization , Chengdu, China., Wu X; Sichuan Provincial Key Laboratory of Animal Breeding and Genetics, Sichuan Animal Science Academy , Chengdu, China., Yu J; Sichuan Provincial Key Laboratory of Animal Breeding and Genetics, Sichuan Animal Science Academy , Chengdu, China., Zhang B; College of Animal & Veterinary Sciences, Southwest Minzu University , Chengdu, China.; Key Laboratory of Ministry of Education and Sichuan Province for Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization , Chengdu, China.
Jazyk:	angličtina
Zdroj:	Microbiology spectrum [Microbiol Spectr] 2024 Jan 11; Vol. 12 (1), pp. e0240323. Date of Electronic Publication: 2023 Dec 04.
DOI:	10.1128/spectrum.02403-23
Abstrakt:	Importance: Porcine epidemic diarrhea (PED) is a highly infectious and economically significant gastrointestinal disorder that affects pigs of all ages. Preventing and controlling PED is achieved by immunizing sows with vaccines, enabling passive piglet immunization via colostrum. The prevalence of G2b porcine epidemic diarrhea virus (PEDV) continues in China despite the use of commercial vaccines, raising questions regarding current vaccine efficacy and the need for novel vaccine development. Adenovirus serotype 5 (Ad5) has several advantages, including high transduction efficiency, a wide range of host cells, and the ability to infect cells at various stages. In this study, we expressed the immunogenic proteins of spike (S) using an Ad5 vector and generated a PED vaccine candidate by inducing significant humoral immunity. The rAd5-PEDV-S prevented PED-induced weight loss, diarrhea, and intestinal damage in piglets. This novel vaccine candidate strain possesses the potential for use in the pig breeding industry. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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