BM-MSCs alleviate diabetic nephropathy in male rats by regulating ER stress, oxidative stress, inflammation, and apoptotic pathways.

Autor:	Khamis T; Department of Pharmacology and Laboratory of Biotechnology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt., Abdelkhalek A; Faculty of Veterinary Medicine, Badr University in Cairo, Badr, Egypt., Abdellatif H; Department of Human and Clinical Anatomy, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat, Oman.; Anatomy and Embryology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Dwidar N; Faculty of Veterinary Medicine, Badr University in Cairo, Badr, Egypt., Said A; Faculty of Veterinary Medicine, Badr University in Cairo, Badr, Egypt., Ahmed R; Faculty of Veterinary Medicine, Badr University in Cairo, Badr, Egypt., Wagdy K; Faculty of Veterinary Medicine, Badr University in Cairo, Badr, Egypt., Elgarhy R; Faculty of Veterinary Medicine, Badr University in Cairo, Badr, Egypt., Eltahan R; Faculty of Veterinary Medicine, Badr University in Cairo, Badr, Egypt., Mohamed H; Faculty of Veterinary Medicine, Badr University in Cairo, Badr, Egypt., Said Amer E; Faculty of Veterinary Medicine, Badr University in Cairo, Badr, Egypt., Hanna M; Faculty of Veterinary Medicine, Badr University in Cairo, Badr, Egypt., Ragab T; Faculty of Veterinary Medicine, Badr University in Cairo, Badr, Egypt., Kishk A; Faculty of Veterinary Medicine, Badr University in Cairo, Badr, Egypt., Wael J; Faculty of Veterinary Medicine, Badr University in Cairo, Badr, Egypt., Sarhan E; Faculty of Veterinary Medicine, Badr University in Cairo, Badr, Egypt., Saweres L; Faculty of Veterinary Medicine, Badr University in Cairo, Badr, Egypt., Reda M; Faculty of Veterinary Medicine, Badr University in Cairo, Badr, Egypt., Elkomy S; Faculty of Veterinary Medicine, Badr University in Cairo, Badr, Egypt., Mohamed A; Faculty of Veterinary Medicine, Badr University in Cairo, Badr, Egypt., Samy A; Faculty of Veterinary Medicine, Badr University in Cairo, Badr, Egypt., Khafaga A; Faculty of Veterinary Medicine, Badr University in Cairo, Badr, Egypt., Shaker Y; Faculty of Veterinary Medicine, Badr University in Cairo, Badr, Egypt., Yehia H; Faculty of Veterinary Medicine, Badr University in Cairo, Badr, Egypt., Alanazi A; College of Medicine, King Saud Bin Abdulaziz University for Health Sciences (KSAU-HS), Riyadh, Saudi Arabia.; King Abdullah International Medical Research Center, Riyadh, Saudi Arabia., Alassiri M; King Abdullah International Medical Research Center, Riyadh, Saudi Arabia.; Department of Basic Sciences, College of Science and Health Professions, King Saud Bin Abdulaziz University for Health Sciences (KSAU-HS), Riyadh, Saudi Arabia.; Department of Pathology and Laboratory Medicine, King Abdulaziz Medical City (KAMC), Ministry of the National Guard-Health Affairs, Riyadh, Saudi Arabia., Tîrziu E; Department of Animal Production and Veterinary Public Health, Faculty of Veterinary Medicine, University of Life Sciences, 'King Mihai I' from Timisoara [ULST], Timisoara, Romania., Bucur IM; Department of Animal Production and Veterinary Public Health, Faculty of Veterinary Medicine, University of Life Sciences, 'King Mihai I' from Timisoara [ULST], Timisoara, Romania., Arisha AH; Department of Animal Physiology and Biochemistry, Faculty of Veterinary Medicine, Badr University in Cairo, Badr, Egypt.; Department of Physiology, Laboratory of Biotechnology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Jazyk:	angličtina
Zdroj:	Frontiers in pharmacology [Front Pharmacol] 2023 Nov 16; Vol. 14, pp. 1265230. Date of Electronic Publication: 2023 Nov 16 (Print Publication: 2023).
DOI:	10.3389/fphar.2023.1265230
Abstrakt:	Introduction: Diabetic nephropathy (DN), a chronic kidney disease, is a major cause of end-stage kidney disease worldwide. Mesenchymal stem cells (MSCs) have become a promising option to mitigate several diabetic complications. Methods: In this study, we evaluated the therapeutic potential of bone marrow-derived mesenchymal stem cells (BM-MSCs) in a rat model of STZ-induced DN. After the confirmation of diabetes, rats were treated with BM-MSCs and sacrificed at week 12 after treatment. Results: Our results showed that STZ-induced DN rats had extensive histopathological changes, significant upregulation in mRNA expression of renal apoptotic markers, ER stress markers, inflammatory markers, fibronectin, and intermediate filament proteins, and reduction of positive immunostaining of PCNA and elevated P53 in kidney tissue compared to the control group. BM-MSC therapy significantly improved renal histopathological changes, reduced renal apoptosis, ER stress, inflammation, and intermediate filament proteins, as well as increased positive immunostaining of PCNA and reduced P53 in renal tissue compared to the STZ-induced DN group. Conclusion: In conclusion, our study indicates that BM-MSCs may have therapeutic potential for the treatment of DN and provide important insights into their potential use as a novel therapeutic approach for DN. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Khamis, Abdelkhalek, Abdellatif, Dwidar, Said, Ahmed, Wagdy, Elgarhy, Eltahan, Mohamed, Said Amer, Hanna, Ragab, Kishk, Wael, Sarhan, Saweres, Reda, Elkomy, Mohamed, Samy, Khafaga, Shaker, Yehia, Alanazi, Alassiri, Tîrziu, Bucur and Arisha.)
Databáze:	MEDLINE
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