Production of monoclonal antibodies to salinomycin.

Autor: Miller GA, Clark RC, Jessee EJ
Jazyk: angličtina
Zdroj: Hybridoma [Hybridoma] 1986 Winter; Vol. 5 (4), pp. 353-60.
DOI: 10.1089/hyb.1986.5.353
Abstrakt: Protein conjugates of salinomycin were prepared and utilized to produce murine monoclonal antibodies (MAbs) in the BALB/c-P3X63.Ag 8.653 fusion system. Several antibodies were cloned, stabilized, and evaluated for cross-reactivity to other ionophorous antibiotics. Selected antibodies were used to develop quantitative assays for salinomycin by means of an enzyme-linked immunosorbent assay (ELISA). These assays were sensitive to 50 ng of salinomycin.
Databáze: MEDLINE