Visualization and quantitation of spine deformity in zebrafish models of scoliosis by micro-computed tomography.
| Autor: | Bearce EA; Institute of Molecular Biology, Department of Biology, University of Oregon, Eugene, OR 97403, USA. Electronic address: bearceea@uoregon.edu., Ricamona BTB; Institute of Molecular Biology, Department of Biology, University of Oregon, Eugene, OR 97403, USA., Fisher KH; Institute of Molecular Biology, Department of Biology, University of Oregon, Eugene, OR 97403, USA., O'Hara-Smith JR; Institute of Molecular Biology, Department of Biology, University of Oregon, Eugene, OR 97403, USA., Grimes DT; Institute of Molecular Biology, Department of Biology, University of Oregon, Eugene, OR 97403, USA. Electronic address: dtgrimes@uoregon.edu. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2023 Dec 15; Vol. 4 (4), pp. 102739. Date of Electronic Publication: 2023 Dec 02. |
| DOI: | 10.1016/j.xpro.2023.102739 |
| Abstrakt: | Zebrafish (Danio rerio) are increasingly used to investigate spine development, growth, and for studying the etiology of spinal deformity, such as scoliosis. Here, we present a micro-computed tomography-based pipeline for visualizing the zebrafish skeleton. We describe steps for sample preparation, imaging, data management, and processing. We then detail analysis of vertebral and spine morphology using open-source software. This protocol will be useful for scientists using zebrafish to understand spine development and disease. For complete details on the use and execution of this protocol, please refer to Bearce et al. (2022). 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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