Isolation and Characterization of Antimicrobial Peptides Isolated from Brevibacillus halotolerans 7WMA2 for the Activity Against Multidrug-Resistant Pathogens.

Autor: Le Han H; The University of Danang, University of Science and Technology, 54 Nguyen Luong Bang St., Danang, 550000, Viet Nam. hlhan@dut.udn.vn., Pham PTV; Faculty of Medicine, Dong A University, Danang, 550000, Viet Nam., Kim SG; Biological Resource Center/Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, Jeongeup, 56212, Republic of Korea.; University of Science and Technology (UST), Daejeon, 34113, Republic of Korea., Chan SS; Institut Sains Biologi, Fakulti Sains, Universiti Malaya, 50603, Kuala Lumpur, Malaysia., Khoo KS; Department of Chemical Engineering and Material Science, Yuan Ze University, Taoyuan, Taiwan. kuanshiong.khoo@hotmail.com., Chew KW; School of Chemistry, Chemical Engineering and Biotechnology, Nanyang Technological University, 62 Nanyang Drive, Singapore, 637459, Singapore., Show PL; Department of Chemical Engineering, Khalifa University, Shakhbout Bin Sultan St, Zone 1, Abu Dhabi, United Arab Emirates., Tran TNT; The University of Danang, University of Technology and Education, 48 Cao Thang st, Danang, 550000, Viet Nam., Nguyen HTV; Faculty of Food, Dong A University, Danang, 550000, Viet Nam., Nguyen PTD; The University of Danang, University of Technology and Education, 48 Cao Thang st, Danang, 550000, Viet Nam. ntdphuong@ute.udn.vn.
Jazyk: angličtina
Zdroj: Molecular biotechnology [Mol Biotechnol] 2024 Dec; Vol. 66 (12), pp. 3618-3627. Date of Electronic Publication: 2023 Dec 02.
DOI: 10.1007/s12033-023-00963-0
Abstrakt: Multidrug resistance to pathogens has posed a severe threat to public health. The threat could be addressed by antimicrobial peptides (AMPs) with broad-spectrum suppression. In this study, Brevibacillus halotolerans 7WMA2, isolated from marine sediment, produced AMPs against Gram-positive and Gram-negative bacteria. The AMPs were precipitated by ammonium sulfate 30% (w/v) from culture broth and dialyzed by a 1 kDa membrane. Tryptone Soy Agar (TSA) was used for the cultivation and resulted in the largest bacteria-inhibiting zones under aerobic conditions at 25 °C, 48 h. An SDS-PAGE gel overlay test revealed that strain 7WMA2 could produce AMPs of 5-10 kDa and showed no degradation when held at 121 °C for 30 min at a wide pH 2-12 range. The AMPs did not cause toxicity to HeLa cells with concentrations up to 500 µg/mL while increasing the arbitrary unit up to eight times. The study showed that the AMPs produced were unique, with broad-spectrum antimicrobial ability.
Competing Interests: Declarations Conflict of interest The authors declared that there are no competing interests.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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