Systematic characterization of the chemical constituents in vitro and in vivo of Qianghuo by UPLC-Q-TOF-MS/MS.

Autor: Wu MR; School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, China., Tang LH; School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, China., Chen YY; School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, China., Shu LX; School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, China., Xu YY; School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, China., Yao YQ; School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, China. Electronic address: yaopharmacy@163.com., Li YB; School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Jazyk: angličtina
Zdroj: Fitoterapia [Fitoterapia] 2024 Jan; Vol. 172, pp. 105758. Date of Electronic Publication: 2023 Dec 01.
DOI: 10.1016/j.fitote.2023.105758
Abstrakt: The Chinese herb Qianghuo is an antiphlogistic herb with many effects and complex components. In this study, the chemical composition of Qianghuo and its components in rat plasma after oral administration were investigated using ultra-high-performance liquid chromatography coupled with quadrupole-time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS). The extracts, blank plasma, and plasma containing the drug were analyzed by mass spectrometry, and data collected in both positive and negative ion modes were analyzed using Masslynx software, and the structures were confirmed by combining the compound fragment ions and mass spectrometry cleavage pathways. A total of 62 in vitro chemical components were identified, including 27 coumarins, 18 organic acids, 5 amino acids, 5 glycosides, 2 flavonoids, 4 nucleotides, and 1 ester, which were summarized from the obtained compounds in terms of their possible cleavage patterns. Among the identified 31 compounds in rat plasma, 21 were prototypes, mostly coumarins, organic acids, and flavonoids, and 10 were metabolites, which were mainly generated via hydroxylation and methylation pathways. Based on these, this study provides a theoretical foundation for quality control and basic research on Qianghuo medicinal substances.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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