Fast screening using attenuated total reflectance- fourier transform infrared (ATR-FTIR) spectroscopy of patients based on D-dimer threshold value.

Autor: Brun BF; Department of Physiological Science, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil., Nascimento MHC; Exact Sciences Center, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil., Dias PAC; Department of Physiological Science, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil., Marcarini WD; Department of Physiological Science, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil; Centro Universitário Vale do CRICARÉ, São Matheus, Espírito Santo, Brazil., Singh MN; Biocel UK Ltd, Hull, HU10 6TS, UK; Chesterfield Royal Hospital, Chesterfield Road, Calow, Chesterfield, S44 5BL, UK., Filgueiras PR; Exact Sciences Center, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil., Vassallo PF; Clinical Hospital, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Romão W; Exact Sciences Center, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil; Federal Institute of Education Science and Technology of Espírito Santo, Vila Velha, Espírito Santo, Brazil., Mill JG; Department of Physiological Science, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil., Martin FL; Biocel UK Ltd, Hull, HU10 6TS, UK; Department of Cellular Pathology, Blackpool Teaching Hospitals NHS Foundation Trust, Whinney Heys Road, Blackpool, FY3 8NR, UK., Barauna VG; Department of Physiological Science, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil. Electronic address: valerio.barauna@ufes.br.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2024 Mar 01; Vol. 269, pp. 125482. Date of Electronic Publication: 2023 Nov 28.
DOI: 10.1016/j.talanta.2023.125482
Abstrakt: Attenuated Total Reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy is an emerging technology in the medical field. Blood D-dimer was initially studied as a marker of the activation of coagulation and fibrinolysis. It is mainly used as a potential diagnosis screening test for pulmonary embolism or deep vein thrombosis but was recently associated with COVID-19 severity. This study aimed to evaluate the use of ATR-FTIR spectroscopy with machine learning to classify plasma D-dimer concentrations. The plasma ATR-FTIR spectra from 100 patients were studied through principal component analysis (PCA) and two supervised approaches: genetic algorithm with linear discriminant analysis (GA-LDA) and partial least squares with linear discriminant (PLS-DA). The spectra were truncated to the fingerprint region (1800-1000 cm -1 ). The GA-LDA method effectively classified patients according to D-dimer cutoff (≤0.5 μg/mL and >0.5 μg/mL) with 87.5 % specificity and 100 % sensitivity on the training set, and 85.7 % specificity, and 95.6 % sensitivity on the test set. Thus, we demonstrate that ATR-FTIR spectroscopy might be an important additional tool for classifying patients according to D-dimer values. ATR-FTIR spectral analyses associated with clinical evidence can contribute to a faster and more accurate medical diagnosis, reduce patient morbidity, and save resources and demand for professionals.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: The co-authors Maneesh N. Singh and Francis L. de Martin have old positions and shareholdings in Biocel UK Ltd. and its subsidiary companies.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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