Developmental stages and episode-specific regulatory genes in andromonoecious melon flower development.

Autor:	Pechar GS; Centro de Edafología y Biología Aplicada del Segura (CEBAS)-CSIC, Department of Stress Biology and Plant Pathology, PO Box 164, 30100 Espinardo, Murcia, Spain., Sánchez-Pina MA; Centro de Edafología y Biología Aplicada del Segura (CEBAS)-CSIC, Department of Stress Biology and Plant Pathology, PO Box 164, 30100 Espinardo, Murcia, Spain., Coronado-Parra T; Microscopy Core Facility, Área Científica y Técnica de Investigación, Universidad de Murcia, PO Box 164, 30100 Espinardo, Murcia, Spain., Bretó P; Abiopep S.L., R&D Department, Parque Científico de Murcia, Ctra. de Madrid, Km 388, Complejo de Espinardo, Edf. R, 2º, 30100 Espinardo, Murcia, Spain., García-Almodóvar RC; Abiopep S.L., R&D Department, Parque Científico de Murcia, Ctra. de Madrid, Km 388, Complejo de Espinardo, Edf. R, 2º, 30100 Espinardo, Murcia, Spain., Liu L; Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Zhengzhou 450009, Henan, China., Aranda MA; Centro de Edafología y Biología Aplicada del Segura (CEBAS)-CSIC, Department of Stress Biology and Plant Pathology, PO Box 164, 30100 Espinardo, Murcia, Spain., Donaire L; Centro de Edafología y Biología Aplicada del Segura (CEBAS)-CSIC, Department of Stress Biology and Plant Pathology, PO Box 164, 30100 Espinardo, Murcia, Spain.; Abiopep S.L., R&D Department, Parque Científico de Murcia, Ctra. de Madrid, Km 388, Complejo de Espinardo, Edf. R, 2º, 30100 Espinardo, Murcia, Spain.
Jazyk:	angličtina
Zdroj:	Annals of botany [Ann Bot] 2024 Apr 10; Vol. 133 (2), pp. 305-320.
DOI:	10.1093/aob/mcad186
Abstrakt:	Background and Aims: Given the lack of specific studies on floral development in melon (Cucumis melo L.), we carried out an extensive study involving morphological and transcriptomic analyses to characterize floral development in this species. Methods: Using an andromonoecious line, we analysed the development of floral buds in male and hermaphrodite flowers with both light microscopy and scanning electron microscopy. Based on flower lengths, we established a correlation between the developmental stages and four main episodes of floral development and conducted an extensive RNA sequencing analysis of these episodes. Key Results: We identified 12 stages of floral development, from the appearance of the floral meristems to anthesis. The main structural differences between male and hermaphrodite flowers appeared between stages 6 and 7; later stages of development leading to the formation of organs and structures in both types of flowers were also described. We analysed the gene expression patterns of the four episodes in flower development to find the genes that were specific to each given episode. Among others, we identified genes that defined the passage from one episode to the next according to the ABCDE model of floral development. Conclusions: This work combines a detailed morphological analysis and a comprehensive transcriptomic study to enable characterization of the structural and molecular mechanisms that determine the floral development of an andromonoecious genotype in melon. Taken together, our results provide a first insight into gene regulation networks in melon floral development that are crucial for flowering and pollen formation, highlighting potential targets for genetic manipulation to improve crop yield of melon in the future. (© The Author(s) 2023. Published by Oxford University Press on behalf of the Annals of Botany Company.)
Databáze:	MEDLINE
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