The role of MoS 2 QDs coated with DSPE-PEG-TPP in the protection of protein secondary structure of the brain tissues in an Alzheimer's disease model.

Autor: Alamri OA; Department of Medical Laboratory, King Fahad Armed Forces Hospital, Jeddah 23311, Saudi Arabia; Department of Biochemistry Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia., Qusti S; Department of Biochemistry Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia., Balgoon M; Department of Biochemistry Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia., Ageeli AA; Department of Chemistry, Faculty of Science, Jazan University, Jazan 45142, Saudi Arabia., Al-Marhaby FA; Department of Physics, Al-Qunfudhah University College, Umm Al-Qura University, Makkah 24230, Saudi Arabia., Alosaimi AM; Department of Chemistry, College of Science, Taif University, Taif 21944, Saudi Arabia., Jowhari MA; Medical Physics Department, Jazan Specialized Hospital, Ministry of Health, Jazan Health Affairs, Jazan 45142, Saudi Arabia., Saeed A; Department of Physics, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia; Department of Physics, Thamar University, Thamar 87246, Yemen. Electronic address: Abdusaeed@tu.ed.ye.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Jan; Vol. 255, pp. 128522. Date of Electronic Publication: 2023 Nov 29.
DOI: 10.1016/j.ijbiomac.2023.128522
Abstrakt: In this investigation, we have explored the protective capacity of MoS 2 QDs coated with 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethyleneglycol) -2000] (DSPE-PEG) linked with (3-carboxypropyl) triphenylphosphonium-bromide (TPP), on the secondary structure of proteins in Alzheimer's disease (AD)-affected brain tissues. Using a cohort of fifteen male SWR/J mice, we establish three groups: a control group, a second group induced with AD through daily doses of AlCl 3 and D-galactose for 49 consecutive days, and a third group receiving the same AD-inducing doses but treated with DSPE-PEG-TPP-MoS 2 QDs. Brain tissues are meticulously separated from the skull, and their molecular structures are analyzed via FTIR spectroscopy. Employing the curve fitting method on the amide I peak, we delve into the nuances of protein secondary structure. The FTIR analysis reveals a marked increase in β-sheet structures and a concurrent decline in turn and α-helix structures in the AD group in comparison to the control group. Notably, no statistically significant differences emerge between the treated and control mice. Furthermore, multivariate analysis of the FTIR spectral region, encompassing protein amide molecular structures, underscores a remarkable similarity between the treated and normal mice. This study elucidates the potential of DSPE-PEG-TPP-MoS 2 QDs in shielding brain tissue proteins against the pathogenic influences of AD.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023. Published by Elsevier B.V.)
Databáze: MEDLINE
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