Recent advances in genome annotation and synthetic biology for the development of microbial chassis.

Autor: Hamese S; Synthetic Nanobiotechnology and Biomachines Group, Centre for Synthetic Biology and Precision Medicine, Next Generation Health Cluster, CSIR Pretoria, South Africa.; Biotechnology Innovation Centre, Rhodes University, PO Box 94, Makhanda, 6140, South Africa., Mugwanda K; Synthetic Nanobiotechnology and Biomachines Group, Centre for Synthetic Biology and Precision Medicine, Next Generation Health Cluster, CSIR Pretoria, South Africa.; Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa., Takundwa M; Synthetic Nanobiotechnology and Biomachines Group, Centre for Synthetic Biology and Precision Medicine, Next Generation Health Cluster, CSIR Pretoria, South Africa., Prinsloo E; Biotechnology Innovation Centre, Rhodes University, PO Box 94, Makhanda, 6140, South Africa., Thimiri Govinda Raj DB; Synthetic Nanobiotechnology and Biomachines Group, Centre for Synthetic Biology and Precision Medicine, Next Generation Health Cluster, CSIR Pretoria, South Africa. dgovindaraj@csir.co.za.
Jazyk: angličtina
Zdroj: Journal, genetic engineering & biotechnology [J Genet Eng Biotechnol] 2023 Dec 01; Vol. 21 (1), pp. 156. Date of Electronic Publication: 2023 Dec 01.
DOI: 10.1186/s43141-023-00598-3
Abstrakt: This article provides an overview of microbial host selection, synthetic biology, genome annotation, metabolic modeling, and computational methods for predicting gene essentiality for developing a microbial chassis. This article focuses on lactic acid bacteria (LAB) as a microbial chassis and strategies for genome annotation of the LAB genome. As a case study, Lactococcus lactis is chosen based on its well-established therapeutic applications such as probiotics and oral vaccine development. In this article, we have delineated the strategies for genome annotations of lactic acid bacteria. These strategies also provide insights into streamlining genome reduction without compromising the functionality of the chassis and the potential for minimal genome chassis development. These insights underscore the potential for the development of efficient and sustainable synthetic biology systems using streamlined microbial chassis with minimal genomes.
(© 2023. The Author(s).)
Databáze: MEDLINE
Externí odkaz: