The Arabidopsis SNARE complex genes regulate the early stages of pollen-stigma interactions.

Autor: Macgregor SR; Department of Cell & Systems Biology, University of Toronto, Toronto, M5S 3B2, Canada.; Faculty of Science, York University, Toronto, Canada., Beronilla PKS; Department of Cell & Systems Biology, University of Toronto, Toronto, M5S 3B2, Canada., Goring DR; Department of Cell & Systems Biology, University of Toronto, Toronto, M5S 3B2, Canada. d.goring@utoronto.ca.; Centre for the Analysis of Genome Evolution & Function, University of Toronto, Toronto, M5S 3B2, Canada. d.goring@utoronto.ca.
Jazyk: angličtina
Zdroj: Plant reproduction [Plant Reprod] 2024 Sep; Vol. 37 (3), pp. 309-320. Date of Electronic Publication: 2023 Dec 01.
DOI: 10.1007/s00497-023-00488-1
Abstrakt: Key Message: The VAMP721, VAMP722, SYP121, SYP122 and SNAP33 SNAREs are required in the Arabidopsis stigma for pollen hydration, further supporting a role for vesicle trafficking in the stigma's pollen responses. In the Brassicaceae, the process of accepting compatible pollen is a key step in successful reproduction and highly regulated following interactions between the pollen and the stigma. Central to this is the initiation of secretion in the stigma, which is proposed to provide resources to the pollen for hydration and germination and pollen tube growth. Previously, the eight exocyst subunit genes were shown to be required in the Arabidopsis stigma to support these pollen responses. One of the roles of the exocyst is to tether secretory vesicles at the plasma membrane for membrane fusion by the SNARE complex to enable vesicle cargo release. Here, we investigate the role of Arabidopsis SNARE genes in the stigma for pollen responses. Using a combination of different knockout and knockdown SNARE mutant lines, we show that VAMP721, VAMP722, SYP121, SYP122 and SNAP33 are involved in this process. Significant disruptions in pollen hydration were observed following pollination of wildtype pollen on the mutant SNARE stigmas. Overall, these results place the Arabidopsis SNARE complex as a contributor in the stigma for pollen responses and reaffirm the significance of secretion in the stigma to support the pollen-stigma interactions.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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