The fungal pathogen Ustilago maydis targets the maize corepressor RELK2 to modulate host transcription for tumorigenesis.

Autor: Huang L; Institute for Plant Sciences and Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, Cologne, 50674, Germany., Ökmen B; Department of Microbial Interactions, IMIT/ZMBP, University of Tübingen, Tübingen, 72076, Germany., Stolze SC; Protein Mass Spectrometry, Max-Planck Institute for Plant Breeding Research, Cologne, 50829, Germany., Kastl M; Institute for Plant Sciences and Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, Cologne, 50674, Germany.; Department of Oncology, Hematology and Rheumatology, University Hospital Bonn, Bonn, 53127, Germany., Khan M; Department of Plant Pathology, Institute of Crop Science and Resource Conservation (INRES), University of Bonn, Bonn, 53115, Germany., Hilbig D; Institute for Plant Sciences and Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, Cologne, 50674, Germany.; Department of Oncology, Hematology and Rheumatology, University Hospital Bonn, Bonn, 53127, Germany., Nakagami H; Protein Mass Spectrometry, Max-Planck Institute for Plant Breeding Research, Cologne, 50829, Germany.; Basic Immune System of Plants, Max Planck Institute for Plant Breeding Research, Cologne, 50829, Germany., Djamei A; Department of Plant Pathology, Institute of Crop Science and Resource Conservation (INRES), University of Bonn, Bonn, 53115, Germany., Doehlemann G; Institute for Plant Sciences and Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, Cologne, 50674, Germany.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2024 Feb; Vol. 241 (4), pp. 1747-1762. Date of Electronic Publication: 2023 Nov 30.
DOI: 10.1111/nph.19448
Abstrakt: Ustilago maydis is a biotrophic fungus that causes tumor formation on all aerial parts of maize. U. maydis secretes effector proteins during penetration and colonization to successfully overcome the plant immune response and reprogram host physiology to promote infection. In this study, we functionally characterized the U. maydis effector protein Topless (TPL) interacting protein 6 (Tip6). We found that Tip6 interacts with the N-terminus of RELK2 through its two Ethylene-responsive element binding factor-associated amphiphilic repression (EAR) motifs. We show that the EAR motifs are essential for the virulence function of Tip6 and critical for altering the nuclear distribution pattern of RELK2. We propose that Tip6 mimics the recruitment of RELK2 by plant repressor proteins, thus disrupting host transcriptional regulation. We show that a large group of AP2/ERF B1 subfamily transcription factors are misregulated in the presence of Tip6. Our study suggests a regulatory mechanism where the U. maydis effector Tip6 utilizes repressive domains to recruit the corepressor RELK2 to disrupt the transcriptional networks of the host plant.
(© 2023 The Authors New Phytologist © 2023 New Phytologist Foundation.)
Databáze: MEDLINE
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