iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy.

Autor: Louvel V; Department of Molecular and Cellular Biology, University of Geneva, Geneva, Switzerland., Haase R; Department of Microbiology and Molecular medicine, University of Geneva, Geneva, Switzerland., Mercey O; Department of Molecular and Cellular Biology, University of Geneva, Geneva, Switzerland., Laporte MH; Department of Molecular and Cellular Biology, University of Geneva, Geneva, Switzerland., Eloy T; ICube - UMR7357, CNRS, University of Strasbourg, Strasbourg, France., Baudrier É; ICube - UMR7357, CNRS, University of Strasbourg, Strasbourg, France., Fortun D; ICube - UMR7357, CNRS, University of Strasbourg, Strasbourg, France., Soldati-Favre D; Department of Microbiology and Molecular medicine, University of Geneva, Geneva, Switzerland., Hamel V; Department of Molecular and Cellular Biology, University of Geneva, Geneva, Switzerland. virginie.hamel@unige.ch., Guichard P; Department of Molecular and Cellular Biology, University of Geneva, Geneva, Switzerland. paul.guichard@unige.ch.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Nov 30; Vol. 14 (1), pp. 7893. Date of Electronic Publication: 2023 Nov 30.
DOI: 10.1038/s41467-023-43582-8
Abstrakt: Expansion microscopy (ExM) is a highly effective technique for super-resolution fluorescence microscopy that enables imaging of biological samples beyond the diffraction limit with conventional fluorescence microscopes. Despite the development of several enhanced protocols, ExM has not yet demonstrated the ability to achieve the precision of nanoscopy techniques such as Single Molecule Localization Microscopy (SMLM). Here, to address this limitation, we have developed an iterative ultrastructure expansion microscopy (iU-ExM) approach that achieves SMLM-level resolution. With iU-ExM, it is now possible to visualize the molecular architecture of gold-standard samples, such as the eight-fold symmetry of nuclear pores or the molecular organization of the conoid in Apicomplexa. With its wide-ranging applications, from isolated organelles to cells and tissue, iU-ExM opens new super-resolution avenues for scientists studying biological structures and functions.
(© 2023. The Author(s).)
Databáze: MEDLINE
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