Utility of nucleic acid amplification test in the detection of tuberculosis in biological fluids from suspected TB patients in a cardiovascular center in the Philippines.

Autor: Balisan OPR; Division of Laboratory Medicine, Philippine Heart Center, East Avenue, Diliman, Quezon City 0850, Philippines. Electronic address: othaniel.balisan@cghc.edu.ph., Galamay JRT; Division of Pulmonary and Critical Care Medicine, Philippine Heart Center, East Avenue, Diliman, Quezon City 0850, Philippines., Cale-Subia LN; Division of Laboratory Medicine, Philippine Heart Center, East Avenue, Diliman, Quezon City 0850, Philippines., De Luna AM; Division of Laboratory Medicine, Philippine Heart Center, East Avenue, Diliman, Quezon City 0850, Philippines.
Jazyk: angličtina
Zdroj: Acta tropica [Acta Trop] 2024 Jan; Vol. 249, pp. 107078. Date of Electronic Publication: 2023 Nov 28.
DOI: 10.1016/j.actatropica.2023.107078
Abstrakt: Background: TB NAAT is highly sensitive and can therefore be a helpful tool used in confirming M. tuberculosis. In a prospective study, we evaluated the utility of TB NAAT in the detection of tuberculosis in biological fluids from suspected TB patients.
Methods: We compared tuberculosis nucleic acid amplification test and acid-fast bacilli smears with Lowenstein-Jensen culture, from patients with a clinical suspicion of tuberculosis disease. We calculated the sensitivity, specificity, PPV and NPV.
Results: Using the Lowenstein-Jensen culture as the gold standard for detection of Mycobacterium tuberculosis, the TB-NAAT showed sensitivity of 66.67 %, specificity of 93.67 %, and gave a positive predictive value of 44.44 %.
Conclusion: We conclude that the TB-NAAT is a quick and consistent diagnostic test for TB detection. However, due to a comparably lower sensitivity than other previous studies, the utility of TB-NAAT alone may not be sufficient in the screening of TB patients. Likewise, the TB-NAAT cannot detect non-tuberculous mycobacteria, for which additional analysis may be needed.
Competing Interests: Declaration of Competing Interest We wish to confirm that there are no known conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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