Liquid Biopsy in Organ Damage: small extracellular vesicle chip-based assessment of polytrauma.

Autor:	Wang B; Department of Internal Medicine I, University Hospital Bonn of the Rheinische Friedrich-Wilhelms-University, Bonn, Germany., Wöhler A; Department of General, Visceral and Thoracic Surgery, German Armed Forces Central Hospital, Koblenz, Germany., Greven J; Department of Orthopaedics, Trauma and Reconstructive Surgery, University Hospital Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen, Aachen, Germany., Salzmann RJS; Department of Internal Medicine I, University Hospital Bonn of the Rheinische Friedrich-Wilhelms-University, Bonn, Germany., Keller CM; Department of Internal Medicine I, University Hospital Bonn of the Rheinische Friedrich-Wilhelms-University, Bonn, Germany., Tertel T; Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany., Zhao Q; Department of Orthopaedics, Trauma and Reconstructive Surgery, University Hospital Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen, Aachen, Germany., Mert Ü; Department of Orthopaedics, Trauma and Reconstructive Surgery, University Hospital Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen, Aachen, Germany., Horst K; Department of Orthopaedics, Trauma and Reconstructive Surgery, University Hospital Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen, Aachen, Germany., Lupu L; Institute of Clinical and Experimental Trauma Immunology, University Hospital Ulm, Ulm, Germany., Huber-Lang M; Institute of Clinical and Experimental Trauma Immunology, University Hospital Ulm, Ulm, Germany., van Griensven M; Department of Cell Biology-Inspired Tissue Engineering, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, Netherlands., Mollnes TE; Research Laboratory, Nordland Hospital Bodø, Bodø, Norway.; Department of Immunology, Oslo University Hospital, and University of Oslo, Oslo, Norway.; Center of Molecular Inflammation Research, Norwegian University of Science and Technology, Trondheim, Norway., Schaaf S; Department of General, Visceral and Thoracic Surgery, German Armed Forces Central Hospital, Koblenz, Germany., Schwab R; Department of General, Visceral and Thoracic Surgery, German Armed Forces Central Hospital, Koblenz, Germany., Strassburg CP; Department of Internal Medicine I, University Hospital Bonn of the Rheinische Friedrich-Wilhelms-University, Bonn, Germany., Schmidt-Wolf IGH; Department of Integrated Oncology, Center for Integrated Oncology, University Hospital Bonn of the Rheinische Friedrich-Wilhelms-University, Bonn, Germany., Giebel B; Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany., Hildebrand F; Department of Orthopaedics, Trauma and Reconstructive Surgery, University Hospital Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen, Aachen, Germany., Lukacs-Kornek V; Institute of Molecular Medicine and Experimental Immunology, University Hospital Bonn of the Rheinische Friedrich-Wilhelms-University, Bonn, Germany., Willms AG; Institute of Molecular Medicine and Experimental Immunology, University Hospital Bonn of the Rheinische Friedrich-Wilhelms-University, Bonn, Germany.; Department of General and Visceral Surgery, German Armed Forces Hospital, Hamburg, Germany., Kornek MT; Department of Internal Medicine I, University Hospital Bonn of the Rheinische Friedrich-Wilhelms-University, Bonn, Germany.; Department of General, Visceral and Thoracic Surgery, German Armed Forces Central Hospital, Koblenz, Germany.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2023 Nov 15; Vol. 14, pp. 1279496. Date of Electronic Publication: 2023 Nov 15 (Print Publication: 2023).
DOI:	10.3389/fimmu.2023.1279496
Abstrakt:	Background: Despite major advances in medicine, blood-borne biomarkers are urgently needed to support decision-making, including polytrauma. Here, we assessed serum-derived extracellular vesicles (EVs) as potential markers of decision-making in polytrauma. Objective: Our Liquid Biopsy in Organ Damage (LiBOD) study aimed to differentiate polytrauma with organ injury from polytrauma without organ injury. We analysed of blood-borne small EVs at the individual level using a combination of immunocapture and high-resolution imaging. Methods: To this end, we isolated, purified, and characterized small EVs according to the latest Minimal Information for Studies of Extracellular Vesicles (MISEV) guidelines from human blood collected within 24 h post-trauma and validated our results using a porcine polytrauma model. Results: We found that small EVs derived from monocytes CD14 + and CD14 + CD61 + were significantly elevated in polytrauma with organ damage. To be precise, our findings revealed that CD9 + CD14 + and CD14 + CD61 + small EVs exhibited superior performance compared to CD9 + CD61 + small EVs in accurately indicating polytrauma with organ damage, reaching a sensitivity and a specificity of 0.81% and 0.97%, respectively. The results in humans were confirmed in an independent porcine model of polytrauma. Conclusion: These findings suggest that these specific types of small EVs may serve as valuable, non-invasive, and objective biomarkers for assessing and monitoring the severity of polytrauma and associated organ damage. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers at the time of submission. This had no impact on the peer review process or the final decision. (Copyright © 2023 Wang, Wöhler, Greven, Salzmann, Keller, Tertel, Zhao, Mert, Horst, Lupu, Huber-Lang, van Griensven, Mollnes, Schaaf, Schwab, Strassburg, Schmidt-Wolf, Giebel, Hildebrand, Lukacs-Kornek, Willms and Kornek.)
Databáze:	MEDLINE
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