Cascading effects of hypobaric hypoxia on the testis: insights from a single-cell RNA sequencing analysis.

Autor:	Ji YH; Department of Urology, Tangdu Hospital, Air Force Military Medical University, Xi'an, Shanxi, China., Wang LM; Department of Urology, Tangdu Hospital, Air Force Military Medical University, Xi'an, Shanxi, China., Zhang FX; Department of Urology, Tangdu Hospital, Air Force Military Medical University, Xi'an, Shanxi, China., Hou HZ; Department of Urology, Xijing Hospital, Air Force Military Medical University, Xi'an, Shanxi, China., Luo ZR; Department of Urology, Tangdu Hospital, Air Force Military Medical University, Xi'an, Shanxi, China., Xue Q; Department of Urology, Tangdu Hospital, Air Force Military Medical University, Xi'an, Shanxi, China., Shi MM; Department of Urology, Tangdu Hospital, Air Force Military Medical University, Xi'an, Shanxi, China., Jiao Y; Department of Urology, Tangdu Hospital, Air Force Military Medical University, Xi'an, Shanxi, China., Cui D; Department of Urology, Tangdu Hospital, Air Force Military Medical University, Xi'an, Shanxi, China., He DL; Department of Urology, Tangdu Hospital, Air Force Military Medical University, Xi'an, Shanxi, China., Xue W; Department of Urology, Tangdu Hospital, Air Force Military Medical University, Xi'an, Shanxi, China., Wen YQ; Department of Bioinformatics, Institute of Health Service and Transfusion Medicine, Beijing, China., Tang QS; Department of Urology, Tangdu Hospital, Air Force Military Medical University, Xi'an, Shanxi, China., Zhang B; Department of Urology, Tangdu Hospital, Air Force Military Medical University, Xi'an, Shanxi, China.
Jazyk:	angličtina
Zdroj:	Frontiers in cell and developmental biology [Front Cell Dev Biol] 2023 Nov 15; Vol. 11, pp. 1282119. Date of Electronic Publication: 2023 Nov 15 (Print Publication: 2023).
DOI:	10.3389/fcell.2023.1282119
Abstrakt:	Most mammals tolerate exposure to hypobaric hypoxia poorly as it may affect multiple regulatory mechanisms and inhibit cell proliferation, promote apoptosis, limit tissue vascularization, and disrupt the acid-base equilibrium. Here, we quantified the functional state of germ cell development and demonstrated the interaction between the germ and somatic cells via single-cell RNA sequencing (scRNA-seq). The present study elucidated the regulatory effects of hypobaric hypoxia exposure on germ cell formation and sperm differentiation by applying enrichment analysis to genomic regions. Hypobaric hypoxia downregulates the genes controlling granule secretion and organic matter biosynthesis, upregulates tektin 1 (TEKT1) and kinesin family member 2C (KIF2C), and downregulates 60S ribosomal protein 11 (RPL11) and cilia- and flagella-associated protein 206 (CFAP206). Our research indicated that prosaposin-G protein-coupled receptor 37 (PSAP-GPR37) ligands mediate the damage to supporting cells caused by hypobaric hypoxic exposure. The present work revealed that hypoxia injures peritubular myoid (PTM) cells and spermatocytes in the S phase. It also showed that elongating spermatids promote maturation toward the G2 phase and increase their functional reserve for sperm-egg binding. The results of this study provide a theoretical basis for future investigations on prophylactic and therapeutic approaches toward protecting the reproductive system against the harmful effects of hypobaric hypoxic exposure. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Ji, Wang, Zhang, Hou, Luo, Xue, Shi, Jiao, Cui, He, Xue, Wen, Tang and Zhang.)
Databáze:	MEDLINE
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