Two plastidial lysophosphatidic acid acyltransferases differentially mediate the biosynthesis of membrane lipids and triacylglycerols in Phaeodactylum tricornutum.

Autor: You L; Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, 430062, China., Połońska A; Intercollegiate Faculty of Biotechnology of UG and MUG, Gdansk, 80-307, Poland., Jasieniecka-Gazarkiewicz K; Intercollegiate Faculty of Biotechnology of UG and MUG, Gdansk, 80-307, Poland., Richard F; Laboratoire de Physiologie Cellulaire et Végétale, Centre National de la Recherche Scientifique, Commissariat à l'Energie Atomique et aux Energies Alternatives, INRAE, Université Grenoble Alpes, Unité mixte de recherche 5168, IRIG, CEA Grenoble, F-38041, Grenoble, France., Jouhet J; Laboratoire de Physiologie Cellulaire et Végétale, Centre National de la Recherche Scientifique, Commissariat à l'Energie Atomique et aux Energies Alternatives, INRAE, Université Grenoble Alpes, Unité mixte de recherche 5168, IRIG, CEA Grenoble, F-38041, Grenoble, France., Maréchal E; Laboratoire de Physiologie Cellulaire et Végétale, Centre National de la Recherche Scientifique, Commissariat à l'Energie Atomique et aux Energies Alternatives, INRAE, Université Grenoble Alpes, Unité mixte de recherche 5168, IRIG, CEA Grenoble, F-38041, Grenoble, France., Banaś A; Intercollegiate Faculty of Biotechnology of UG and MUG, Gdansk, 80-307, Poland., Hu H; Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China., Pan Y; Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China., Hao X; Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, 430062, China., Jin H; Center for Microalgal Biotechnology and Biofuels, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China., Allen AE; Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA, 92093, USA.; J. Craig Venter Institute, 4120 Capricorn Lane, La Jolla, CA, 92037, USA., Amato A; Laboratoire de Physiologie Cellulaire et Végétale, Centre National de la Recherche Scientifique, Commissariat à l'Energie Atomique et aux Energies Alternatives, INRAE, Université Grenoble Alpes, Unité mixte de recherche 5168, IRIG, CEA Grenoble, F-38041, Grenoble, France., Gong Y; Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, 430062, China.; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, 430062, China.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2024 Feb; Vol. 241 (4), pp. 1543-1558. Date of Electronic Publication: 2023 Nov 29.
DOI: 10.1111/nph.19434
Abstrakt: Lysophosphatidic acid acyltransferases (LPAATs) catalyze the formation of phosphatidic acid (PA), a central metabolite in both prokaryotic and eukaryotic organisms for glycerolipid biosynthesis. Phaeodactylum tricornutum contains at least two plastid-localized LPAATs (ptATS2a and ptATS2b), but their roles in lipid synthesis remain unknown. Both ptATS2a and ptATS2b could complement the high temperature sensitivity of the bacterial plsC mutant deficient in LPAAT. In vitro enzyme assays showed that they prefer lysophosphatidic acid over other lysophospholipids. ptATS2a is localized in the plastid inner envelope membrane and CRISPR/Cas9-generated ptATS2a mutants showed compromised cell growth, significantly changed plastid and extra-plastidial membrane lipids at nitrogen-replete condition and reduced triacylglycerols (TAGs) under nitrogen-depleted condition. ptATS2b is localized in thylakoid membranes and its knockout led to reduced growth rate and TAG content but slightly altered molecular composition of membrane lipids. The changes in glycerolipid profiles are consistent with the role of both LPAATs in the sn-2 acylation of sn-1-acyl-glycerol-3-phosphate substrates harboring 20:5 at the sn-1 position. Our findings suggest that both LPAATs are important for membrane lipids and TAG biosynthesis in P. tricornutum and further highlight that 20:5-Lyso-PA is likely involved in the massive import of 20:5 back to the plastid to feed plastid glycerolipid syntheses.
(© 2023 The Authors New Phytologist © 2023 New Phytologist Foundation.)
Databáze: MEDLINE
Externí odkaz: