| Autor: |
Kariyawasam R; Department of Laboratory Medicine & Pathology, University of Alberta, Edmonton, AB T6G 2R3, Canada.; Alberta Precision Laboratories-Public Health, Edmonton, AB T6G 2J2, Canada., Lau R; Public Health Ontario Laboratory, Toronto, ON M5G 1M1 Canada., Valencia BM; Instituto de Medicina Tropical 'Alejandro von Humboldt', Lima, Peru.; Kirby Institute, University of New South Wales, Sydney, Australia., Llanos-Cuentas A; Instituto de Medicina Tropical 'Alejandro von Humboldt', Lima, Peru., Boggild AK; Department of Medicine, University of Toronto, Toronto, ON M5S 1A8, Canada.; Tropical Disease Unit, Toronto General Hospital, Toronto, ON M5G 2C4, Canada.; Institute of Medical Science, University of Toronto, Toronto, ON M5S 1A8, Canada. |
| Abstrakt: |
American tegumentary leishmaniasis comprises a discrete set of clinical presentations endemic to Latin America. Leishmania RNA virus-1 (LRV-1) is a double-stranded RNA virus identified in 20–25% of the Leishmania Viannia braziliensis and L. V. guyanensis , however not in L. V. panamensis . This is the first report of LRV-1 in L. V. panamensis and its associations with clinical phenotypes of ATL. Unique surplus discard clinical isolates of L. V. panamensis were identified from the Public Health Ontario Laboratory (PHOL) and the Leishmania Clinic of the Instituto de Medicina Tropical ‘Alexander von Humboldt’ between 2012 and 2019 and screened for LRV-1 by real-time polymerase chain reaction. Patient isolates were stratified according to clinical phenotype. Of 30 patients with L. V. panamensis , 14 (47%) and 16 (53%) patients had severe and non-severe ATL, respectively. Five (36%) of 14 severe cases and 2 (12%) of 16 non-severe cases were positive for LRV-1, respectively. No differences in sex were observed for clinical phenotype and LRV-1 status. Although an association between LRV-1 status and clinical phenotype was not demonstrated, this is the first description of the novel detection of LRV-1 in L. V. panamensis , a species that has been documented predominantly in Central America. |
