Role of pre-existing immunity in driving the dengue virus serotype 2 genotype shift in the Philippines: A retrospective analysis of serological data.

Autor:	Balingit JC; Department of Tropical Viral Vaccine Development, Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki City, Nagasaki, Japan; Department of Virology, Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki City, Nagasaki, Japan., Dimamay MPS; Research and Biotechnology Group, St. Luke's Medical Center, Quezon City, Metro Manila, Philippines., Suzuki R; Department of Virology II, National Institute of Infectious Diseases, Gakuen, Musashi-murayama, Tokyo, Japan., Matsuda M; Department of Virology II, National Institute of Infectious Diseases, Gakuen, Musashi-murayama, Tokyo, Japan., Xayavong D; Department of Virology, Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki City, Nagasaki, Japan., Ngwe Tun MM; Department of Tropical Viral Vaccine Development, Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki City, Nagasaki, Japan; Department of Virology, Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki City, Nagasaki, Japan., Matias RR; Research and Biotechnology Group, St. Luke's Medical Center, Quezon City, Metro Manila, Philippines., Natividad FF; National Ethics Committee, Philippine Council for Health Research and Development, Department of Science and Technology (DOST), Saliksik Building, DOST Compound, Bicutan, Taguig City, Metro Manila, Philippines., Moi ML; Department of Developmental Medical Sciences, School of International Health, University of Tokyo, Bunkyo-ku, Tokyo, Japan., Takamatsu Y; Department of Tropical Viral Vaccine Development, Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki City, Nagasaki, Japan; Department of Virology, Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki City, Nagasaki, Japan., Culleton R; Division of Molecular Parasitology, Proteo-Science Center, Ehime University, Shitsukawa, Ehime, Japan., Buerano CC; Department of Tropical Viral Vaccine Development, Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki City, Nagasaki, Japan; Research and Biotechnology Group, St. Luke's Medical Center, Quezon City, Metro Manila, Philippines., Morita K; Department of Tropical Viral Vaccine Development, Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki City, Nagasaki, Japan; Department of Virology, Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki City, Nagasaki, Japan; DEJIMA Infectious Disease Research Alliance, Nagasaki University, Sakamoto, Nagasaki City, Nagasaki, Japan. Electronic address: moritak@nagasaki-u.ac.jp.
Jazyk:	angličtina
Zdroj:	International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases [Int J Infect Dis] 2024 Feb; Vol. 139, pp. 59-68. Date of Electronic Publication: 2023 Nov 27.
DOI:	10.1016/j.ijid.2023.11.025
Abstrakt:	Objective: The invasion of dengue virus (DENV)-2 Cosmopolitan genotype into the Philippines, where the Asian II genotype previously circulated challenges the principle of dengue serotype-specific immunity. Assessment of antibodies in this population may provide a mechanistic basis for how new genotypes emerge in dengue-endemic areas. Methods: We evaluated the neutralizing antibody (nAb) and antibody-dependent enhancement (ADE) responses against the two genotypes using archived serum samples collected from 333 patients with confirmed dengue in Metro Manila, Philippines, before, during, and after the introduction of the Cosmopolitan genotype. We quantified nAb titers in baby hamster kidney (BHK-21) cells with or without the Fcγ receptor IIA (FcγRIIA) to detect the capacity of virus-antibody complexes to neutralize or enhance DENV. Results: The nAb potency of the archived serum samples against the two genotypes was greatly affected by the presence of FcγRIIA. We found significant differences in nAb titers between the two genotypes in BHK-21 cells with FcγRIIA (P <0.0001). The archived serum samples were incapable of fully neutralizing the Cosmopolitan genotype, but instead strongly promoted its ADE compared to the Asian II genotype (P <0.0001). Conclusion: These results reinforce the role of pre-existing immunity in driving genotype shifts. Our finding that specific genotypes exhibit differing susceptibilities to ADE by cross-reactive antibodies may have implications for dengue vaccine development. Competing Interests: Declaration of competing interests The authors have no competing interests to declare. (Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze:	MEDLINE
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