Spherical Fe 2 O 3 nanoparticles inhibit the production of aflatoxins (B 1 and B 2 ) and regulate total soluble solids and titratable acidity of peach fruit.

Autor: Akbar M; Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan., Ali N; Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan., Imran M; Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan., Hussain A; Department of Electronics, Quaid-i-Azam University, Islamabad 45320, Pakistan., Hassan SW; Department of Biosciences, University of Wah, Quaid Avenue, Wah Cantt., Pakistan., Haroon U; Department of Plant Pathology, University of California, Davis 95616, USA., Kamal A; Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan., Farhana; Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan., Chaudhary HJ; Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan., Munis MFH; Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan. Electronic address: munis@qau.edu.pk.
Jazyk: angličtina
Zdroj: International journal of food microbiology [Int J Food Microbiol] 2024 Jan 30; Vol. 410, pp. 110508. Date of Electronic Publication: 2023 Nov 24.
DOI: 10.1016/j.ijfoodmicro.2023.110508
Abstrakt: Aflatoxin is a group I carcinogen and causes significant public health and food safety risks, throughout the world. This study was carried out to assess the levels of aflatoxin contamination in diseased peach (Prunus persica L.) fruit and their control using myco-synthesized iron oxide nanoparticles (Fe 2 O 3 NPs). Diseased peach fruit were diagnosed to be infected with Aspergillus flavus. The isolated pathogen was cultured under UV light (365 nm) and exposed to ammonium hydroxide (31 %) vapors, which confirmed its ability to produce aflatoxin. For the control of this disease, Fe 2 O 3 NPs were synthesized in the filtrate of a biocontrol fungus (Trichoderma harzianum) and characterized before analyzing their potential in disease control. FTIR spectrum described the presence of capping and reducing agents (secondary amines, alcohol, alkyne and aromatic compounds) on the surface of Fe 2 O 3 NPs. X-ray Diffraction (XRD) described the crystalline size (7.78), while the spherical shape of Fe 2 O 3 NPs was described by the SEM analysis. The EDX spectrum indicated the successful formation of Fe 2 O 3 NPs by showing strong signals of iron (74.38 %). All concentrations displayed mycelial growth inhibition, in vitro and the greatest growth reduction (65.4 %) was observed at 1 mg/ml concentration of NPs. At the same concentration of Fe 2 O 3 NPs, significant control of fruit rot of peach was also observed, in vivo. Treatment of Fe 2 O 3 NPs maintained higher soluble solids, sucrose, total sugar, ascorbic acid, titratable acidity and firmness of peach fruit. Diseased fruit were further investigated for the presence and detection of aflatoxins. All three methods viz. thin layer chromatography (TLC), enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC) confirmed a higher production of aflatoxins in control plants, while this production was significantly reduced in Fe 2 O 3 NPs-treated peach fruit.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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