Herbal supplement Spirulina stimulates inflammatory cytokine production in patients with dermatomyositis in vitro .

Autor: Bax CE; Corporal Michael J. Crescenz VA Medical Center, Philadelphia, PA, USA.; Department of Dermatology, University of Pennsylvania, Philadelphia, PA, USA., Diaz D; Corporal Michael J. Crescenz VA Medical Center, Philadelphia, PA, USA.; Department of Dermatology, University of Pennsylvania, Philadelphia, PA, USA., Li Y; Corporal Michael J. Crescenz VA Medical Center, Philadelphia, PA, USA.; Department of Dermatology, University of Pennsylvania, Philadelphia, PA, USA., Vazquez T; Corporal Michael J. Crescenz VA Medical Center, Philadelphia, PA, USA.; Department of Dermatology, University of Pennsylvania, Philadelphia, PA, USA., Patel J; Corporal Michael J. Crescenz VA Medical Center, Philadelphia, PA, USA.; Department of Dermatology, University of Pennsylvania, Philadelphia, PA, USA., Grinnell M; Corporal Michael J. Crescenz VA Medical Center, Philadelphia, PA, USA.; Department of Dermatology, University of Pennsylvania, Philadelphia, PA, USA., Ravishankar A; Corporal Michael J. Crescenz VA Medical Center, Philadelphia, PA, USA.; Department of Dermatology, University of Pennsylvania, Philadelphia, PA, USA., Maddukuri S; Corporal Michael J. Crescenz VA Medical Center, Philadelphia, PA, USA.; Department of Dermatology, University of Pennsylvania, Philadelphia, PA, USA., Keyes E; Corporal Michael J. Crescenz VA Medical Center, Philadelphia, PA, USA.; Department of Dermatology, University of Pennsylvania, Philadelphia, PA, USA., Yan D; Corporal Michael J. Crescenz VA Medical Center, Philadelphia, PA, USA.; Department of Dermatology, University of Pennsylvania, Philadelphia, PA, USA., Bashir M; Corporal Michael J. Crescenz VA Medical Center, Philadelphia, PA, USA.; Department of Dermatology, University of Pennsylvania, Philadelphia, PA, USA., Werth VP; Corporal Michael J. Crescenz VA Medical Center, Philadelphia, PA, USA.; Department of Dermatology, University of Pennsylvania, Philadelphia, PA, USA.
Jazyk: angličtina
Zdroj: IScience [iScience] 2023 Oct 28; Vol. 26 (11), pp. 108355. Date of Electronic Publication: 2023 Oct 28 (Print Publication: 2023).
DOI: 10.1016/j.isci.2023.108355
Abstrakt: Spirulina, an herbal supplement and popular ingredient in health foods, is a potent stimulant of the immune system. Spirulina use is temporally associated with the onset or exacerbation of Dermatomyositis (DM), an autoimmune connective tissue disease that frequently affects the skin and muscle. In this study, we investigated the effect of Spirulina on peripheral blood mononuclear cells (PBMCs) in DM and Healthy Controls (HCs), showing that Spirulina stimulates Interferon β (IFNβ), Tumor necrosis factor α (TNFα), and Interferon γ (IFNγ) production of DM PBMCs primarily via Toll-Like Receptor 4 (TLR4) activation using ELISA (enzyme linked immunosorbent assay) and flow cytometry. We show that classical monocytes and monocyte-derived dendritic cells are stimulated by Spirulina and are activated via TLR4. Skin from patients with Spirulina-associated DM exhibits an inflammatory milieu similar to that of idiopathic DM but with a stronger correlation of TLR4 and IFNγ.
Competing Interests: The authors declare no conflicts of interest.
Databáze: MEDLINE