Changes in neuropeptide large dense core vesicle trafficking dynamics contribute to adaptive responses to a systemic homeostatic challenge.

Autor:	Kirchner MK; Center for Neuroinflammation and Cardiometabolic Diseases, Georgia State University, Atlanta, GA 30303, USA.; Neuroscience Institute, Georgia State University, Atlanta, GA 30303, USA., Althammer F; Center for Neuroinflammation and Cardiometabolic Diseases, Georgia State University, Atlanta, GA 30303, USA.; Institute of Human Genetics, Heidelberg University Hospital, 69120 Heidelberg, Germany., Donaldson KJ; Neuroscience Institute, Georgia State University, Atlanta, GA 30303, USA.; Center for Neuromics, Georgia State University, Atlanta, GA 30303, USA., Cox DN; Neuroscience Institute, Georgia State University, Atlanta, GA 30303, USA.; Center for Neuromics, Georgia State University, Atlanta, GA 30303, USA., Stern JE; Center for Neuroinflammation and Cardiometabolic Diseases, Georgia State University, Atlanta, GA 30303, USA.; Neuroscience Institute, Georgia State University, Atlanta, GA 30303, USA.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2023 Oct 18; Vol. 26 (11), pp. 108243. Date of Electronic Publication: 2023 Oct 18 (Print Publication: 2023).
DOI:	10.1016/j.isci.2023.108243
Abstrakt:	Neuropeptides are packed into large dense core vesicles (LDCVs) that are transported from the soma out into their processes. Limited information exists regarding mechanisms regulating LDCV trafficking, particularly during challenges to bodily homeostasis. Addressing this gap, we used 2-photon imaging in an ex vivo preparation to study LDCVs trafficking dynamics in vasopressin (VP) neurons, which traffic and release neuropeptide from their dendrites and axons. We report a dynamic bidirectional trafficking of VP-LDCVs with important differences in speed and directionality between axons and dendrites. Acute, short-lasting stimuli known to alter VP firing activity and axonal/dendritic release caused modest changes in VP-LDCVs trafficking dynamics. Conversely, chronic/sustained systemic osmotic challenges upregulated VP-LDCVs trafficking dynamic, with a larger effect in dendrites. These results support differential regulation of dendritic and axonal LDCV trafficking, and that changes in trafficking dynamics constitute a novel mechanism by which peptidergic neurons can efficiently adapt to conditions of increased hormonal demand. Competing Interests: The authors declare no competing interests. (© 2023 The Authors.)
Databáze:	MEDLINE
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