Trade-offs constrain adaptive pathways to the type VI secretion system survival.

Autor:	MacGillivray KA; School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, USA.; Parker H. Petit Institute for Bioengineering & Bioscience, Georgia Institute of Technology, Atlanta, GA, USA.; Center for Microbial Dynamics and Infection, Georgia Institute of Technology, Atlanta, GA, USA., Ng SL; School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, USA.; Parker H. Petit Institute for Bioengineering & Bioscience, Georgia Institute of Technology, Atlanta, GA, USA.; Center for Microbial Dynamics and Infection, Georgia Institute of Technology, Atlanta, GA, USA., Wiesenfeld S; School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, USA.; Parker H. Petit Institute for Bioengineering & Bioscience, Georgia Institute of Technology, Atlanta, GA, USA.; Center for Microbial Dynamics and Infection, Georgia Institute of Technology, Atlanta, GA, USA., Guest RL; Department of Molecular Biology, Princeton University, Princeton, NJ, USA., Jubery T; School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, USA.; Parker H. Petit Institute for Bioengineering & Bioscience, Georgia Institute of Technology, Atlanta, GA, USA.; Center for Microbial Dynamics and Infection, Georgia Institute of Technology, Atlanta, GA, USA., Silhavy TJ; Department of Molecular Biology, Princeton University, Princeton, NJ, USA., Ratcliff WC; School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, USA.; Parker H. Petit Institute for Bioengineering & Bioscience, Georgia Institute of Technology, Atlanta, GA, USA.; Center for Microbial Dynamics and Infection, Georgia Institute of Technology, Atlanta, GA, USA., Hammer BK; School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, USA.; Parker H. Petit Institute for Bioengineering & Bioscience, Georgia Institute of Technology, Atlanta, GA, USA.; Center for Microbial Dynamics and Infection, Georgia Institute of Technology, Atlanta, GA, USA.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2023 Oct 26; Vol. 26 (12), pp. 108332. Date of Electronic Publication: 2023 Oct 26 (Print Publication: 2023).
DOI:	10.1016/j.isci.2023.108332
Abstrakt:	The Type VI Secretion System (T6SS) is a nano-harpoon used by many bacteria to inject toxins into neighboring cells. While much is understood about mechanisms of T6SS-mediated toxicity, less is known about the ways that competitors can defend themselves against this attack, especially in the absence of their own T6SS. Here we subjected eight replicate populations of Escherichia coli to T6SS attack by Vibrio cholerae . Over ∼500 generations of competition, isolates of the E. coli populations evolved to survive T6SS attack an average of 27-fold better, through two convergently evolved pathways: apaH was mutated in six of the eight replicate populations, while the other two populations each had mutations in both yejM and yjeP . However, the mutations we identified are pleiotropic, reducing cellular growth rates, and increasing susceptibility to antibiotics and elevated pH. These trade-offs help us understand how the T6SS shapes the evolution of bacterial interactions. Competing Interests: The authors have no conflicts of interest to declare.
Databáze:	MEDLINE
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