Candidate genes for field resistance to cassava brown streak disease revealed through the analysis of multiple data sources.

Autor:	Ferguson ME; Cassava Breeding, International Institute of Tropical Agriculture (IITA), Nairobi, Kenya., Eyles RP; Cassava Breeding, International Institute of Tropical Agriculture (IITA), Nairobi, Kenya., Garcia-Oliveira AL; Cassava Breeding, International Institute of Tropical Agriculture (IITA), Nairobi, Kenya., Kapinga F; Cassava Breeding, International Institute of Tropical Agriculture (IITA), Nairobi, Kenya.; Cassava Breeding, Naliendele Agricultural Research Institute, Mtwara, Tanzania., Masumba EA; Cassava Breeding, International Institute of Tropical Agriculture (IITA), Nairobi, Kenya.; Cassava Breeding, Sugarcane Research Institute, Kibaha, Tanzania., Amuge T; Cassava Breeding, International Institute of Tropical Agriculture (IITA), Nairobi, Kenya.; Cassava Breeding, National Crops Resources Research Institute (NaCRRI), Namulonge, Uganda., Bredeson JV; Molecular and Cell Biology Department, University of California, Berkeley, Berkeley, CA, United States., Rokhsar DS; Molecular and Cell Biology Department, University of California, Berkeley, Berkeley, CA, United States., Lyons JB; Molecular and Cell Biology Department, University of California, Berkeley, Berkeley, CA, United States., Shah T; Bioinformatics, International Institute of Tropical Agriculture (IITA), Nairobi, Kenya., Rounsley S; Seeds & Traits R&D, Dow AgroSciences, Indianapolis, IN, United States., Mkamilo G; Cassava Breeding, Naliendele Agricultural Research Institute, Mtwara, Tanzania.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2023 Nov 03; Vol. 14, pp. 1270963. Date of Electronic Publication: 2023 Nov 03 (Print Publication: 2023).
DOI:	10.3389/fpls.2023.1270963
Abstrakt:	Cassava ( Manihot esculenta Crantz) is a food and industrial storage root crop with substantial potential to contribute to managing risk associated with climate change due to its inherent resilience and in providing a biodegradable option in manufacturing. In Africa, cassava production is challenged by two viral diseases, cassava brown streak disease (CBSD) and cassava mosaic disease. Here we detect quantitative trait loci (QTL) associated with CBSD in a biparental mapping population of a Tanzanian landrace, Nachinyaya and AR37-80, phenotyped in two locations over three years. The purpose was to use the information to ultimately facilitate either marker-assisted selection or adjust weightings in genomic selection to increase the efficiency of breeding. Results from this study were considered in relation to those from four other biparental populations, of similar genetic backgrounds, that were phenotyped and genotyped simultaneously. Further, we investigated the co-localization of QTL for CBSD resistance across populations and the genetic relationships of parents based on whole genome sequence information. Two QTL on chromosome 4 for resistance to CBSD foliar symptoms and one on each of chromosomes 11 and 18 for root necrosis were of interest. Of significance within the candidate genes underlying the QTL on chromosome 4 are Phenylalanine ammonia-lyase (PAL) and Cinnamoyl-CoA reductase (CCR) genes and three PEPR1-related kinases associated with the lignin pathway. In addition, a CCR gene was also underlying the root necrosis-resistant QTL on chromosome 11. Upregulation of key genes in the cassava lignification pathway from an earlier transcriptome study, including PAL and CCR, in a CBSD-resistant landrace compared to a susceptible landrace suggests a higher level of basal lignin deposition in the CBSD-resistant landrace. Earlier RNAscope ® in situ hybridisation imaging experiments demonstrate that cassava brown streak virus (CBSV) is restricted to phloem vessels in CBSV-resistant varieties, and phloem unloading for replication in mesophyll cells is prevented. The results provide evidence for the involvement of the lignin pathway. In addition, five eukaryotic initiation factor (eIF) genes associated with plant virus resistance were found within the priority QTL regions. Competing Interests: Author SR was employed by the company Dow AgroSciences. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Ferguson, Eyles, Garcia-Oliveira, Kapinga, Masumba, Amuge, Bredeson, Rokhsar, Lyons, Shah, Rounsley and Mkamilo.)
Databáze:	MEDLINE
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