Gamma-interferon assay for the ancillary diagnosis of bovine tuberculosis in dairy cattle in urban and adjacent areas of Dhaka city, Bangladesh.
| Autor: | Rumi TB; International Center for Diarrhoeal Disease Research, Dhaka, Bangladesh., Islam SS; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.; Department of Livestock Services, Ministry of Fisheries and Livestock, Dhaka-1215, Bangladesh., Islam R; Department of Microbiology, Jagannath University, Dhaka-1100, Bangladesh., Faisal MMH; International Center for Diarrhoeal Disease Research, Dhaka, Bangladesh., Kabir SML; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh., Rahman AKMA; Department of Medicine, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh., Rahim Z; International Center for Diarrhoeal Disease Research, Dhaka, Bangladesh. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Veterinary world [Vet World] 2023 Oct; Vol. 16 (10), pp. 2120-2127. Date of Electronic Publication: 2023 Oct 17. |
| DOI: | 10.14202/vetworld.2023.2120-2127 |
| Abstrakt: | Background and Aim: Bovine tuberculosis (bTB) is an infectious disease of cattle, mainly caused by Mycobacterium bovis. This study aimed to evaluate the efficacy of the interferon-gamma (IFN-γ) assay and single-intradermal comparative tuberculin test (SICTT) in detecting bTB. Materials and Methods: In an earlier study, 150 positive, 83 inconclusive, and 480 negative animals from 24 cattle herds were screened using SICTT. From these groups, 125 positive, 17 inconclusive, and six negative animals were subsequently verified using the IFN-γ assay. Single-intradermal comparative tuberculin test outcomes were interpreted according to standard guidelines, whereas blood samples were collected and stimulated with purified protein derivatives. Sandwich enzyme-linked immunosorbent assay was used to measure secreted IFN-γ. Concordant and Bayesian latent class analyses were performed to evaluate test performance. Results: Results from the IFN-γ assay revealed that 83.2%, 64.7%, and 16.67% of the animals were positive in the SICTT-positive, inconclusive, and negative animal categories, respectively. Sensitivity (SE) and specificity (SP) of SICTT were 83.9% (95% confidence interval [CI]: 77.4-90.1) and 95.7% (95% CI: 86.9-99.7), respectively. Sensitivity and SP for the IFN-γ assay were 78.9% (95% CI: 71.9-85.4) and 83.9% (65.9-95.9), respectively. The use of both tests in parallel increases the SE of bTB detection (~94%), compared with SICTT alone. Conclusion: Use of the IFN-γ assay with SICTT in parallel, predominantly on cattle demonstrating an inconclusive SICTT outcome, boosts bTB detection rate in low resource settings. Competing Interests: The authors declare that they have no competing interests. (Copyright: © Rumi, et al.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|