Quantification of prefrontal cortical neuronal ensembles following conditioned fear learning in a Fos-LacZ transgenic rat with photoacoustic imaging in Vivo .

Autor:	Matchynski JI; Department of Psychiatry and Behavioral Neurosciences, Wayne State University School of Medicine, Detroit, MI, USA.; Translational Neuroscience Program, Wayne State University School of Medicine, Detroit, MI, USA.; John D. Dingell Veterans Affairs Medical Center, Detroit, MI, USA.; Wayne State MD/PhD Program, Wayne State University School of Medicine, Detroit, MI, USA., Cilley TS; Department of Psychiatry and Behavioral Neurosciences, Wayne State University School of Medicine, Detroit, MI, USA., Sadik N; Department of Psychiatry and Behavioral Neurosciences, Wayne State University School of Medicine, Detroit, MI, USA., Makki KM; John D. Dingell Veterans Affairs Medical Center, Detroit, MI, USA., Wu M; John D. Dingell Veterans Affairs Medical Center, Detroit, MI, USA., Manwar R; University of Illinois at Chicago, Department of Bioengineering, Chicago, IL, USA., Woznicki AR; John D. Dingell Veterans Affairs Medical Center, Detroit, MI, USA., Kallakuri S; Department of Psychiatry and Behavioral Neurosciences, Wayne State University School of Medicine, Detroit, MI, USA., Arfken CL; Department of Psychiatry and Behavioral Neurosciences, Wayne State University School of Medicine, Detroit, MI, USA.; Translational Neuroscience Program, Wayne State University School of Medicine, Detroit, MI, USA., Hope BT; The National Institute on Drug Abuse (NIDA), Intramural Research Program, Baltimore, MD, USA., Avanaki K; University of Illinois at Chicago, Department of Bioengineering, Chicago, IL, USA., Conti AC; Department of Psychiatry and Behavioral Neurosciences, Wayne State University School of Medicine, Detroit, MI, USA.; Translational Neuroscience Program, Wayne State University School of Medicine, Detroit, MI, USA.; John D. Dingell Veterans Affairs Medical Center, Detroit, MI, USA., Perrine SA; Department of Psychiatry and Behavioral Neurosciences, Wayne State University School of Medicine, Detroit, MI, USA.; Translational Neuroscience Program, Wayne State University School of Medicine, Detroit, MI, USA.
Jazyk:	angličtina
Zdroj:	Photoacoustics [Photoacoustics] 2023 Aug 27; Vol. 33, pp. 100551. Date of Electronic Publication: 2023 Aug 27 (Print Publication: 2023).
DOI:	10.1016/j.pacs.2023.100551
Abstrakt:	Understanding the neurobiology of complex behaviors requires measurement of activity in the discrete population of active neurons, neuronal ensembles, which control the behavior. Conventional neuroimaging techniques ineffectively measure neuronal ensemble activity in the brain in vivo because they assess the average regional neuronal activity instead of the specific activity of the neuronal ensemble that mediates the behavior. Our functional molecular photoacoustic tomography (FM-PAT) system allows direct imaging of Fos-dependent neuronal ensemble activation in Fos-LacZ transgenic rats in vivo. We tested four experimental conditions and found increased FM-PAT signal in prefrontal cortical areas in rats undergoing conditioned fear or novel context exposure. A parallel immunofluorescence ex vivo study of Fos expression found similar findings. These findings demonstrate the ability of FM-PAT to measure Fos-expressing neuronal ensembles directly in vivo and support a mechanistic role for the prefrontal cortex in higher-order processing of response to specific stimuli or environmental cues. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2023 The Authors.)
Databáze:	MEDLINE
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