Time domain diffuse Raman spectroscopy using single pixel detection.

Autor:	Bossi A; Politecnico di Milano, Department of Physics, Milan, Italy.; Politecnico di Milano, Department of Mechanics, Milan, Italy., Sekar SKV; IPIC, Tyndall National Institute, Cork, Ireland., Lacerenza M; Politecnico di Milano, Department of Physics, Milan, Italy.; PIONIRS s.r.l. Milano, Italy., Gandolfi V; Politecnico di Milano, Department of Physics, Milan, Italy., Šušnjar S; SpectraCure AB, Gasverksgatan 1, SE-222 29 Lund, Sweden.; Department of Physics, Lund University, P.O. Box 118, SE-221 00 Lund, Sweden., Lanka P; IPIC, Tyndall National Institute, Cork, Ireland., D'Andrea C; Politecnico di Milano, Department of Physics, Milan, Italy., Vanna R; CNR-Institute for Photonics and Nanotechnologies (CNR-IFN), Milan, Italy., Valentini G; Politecnico di Milano, Department of Physics, Milan, Italy.; CNR-Institute for Photonics and Nanotechnologies (CNR-IFN), Milan, Italy., Farina A; CNR-Institute for Photonics and Nanotechnologies (CNR-IFN), Milan, Italy., Pifferi A; Politecnico di Milano, Department of Physics, Milan, Italy.; CNR-Institute for Photonics and Nanotechnologies (CNR-IFN), Milan, Italy.
Jazyk:	angličtina
Zdroj:	Biomedical optics express [Biomed Opt Express] 2023 Oct 13; Vol. 14 (11), pp. 5749-5763. Date of Electronic Publication: 2023 Oct 13 (Print Publication: 2023).
DOI:	10.1364/BOE.502022
Abstrakt:	Diffuse Raman spectroscopy (DIRS) extends the high chemical specificity of Raman scattering to in-depth investigation of thick biological tissues. We present here a novel approach for time-domain diffuse Raman spectroscopy (TD-DIRS) based on a single-pixel detector and a digital micromirror device (DMD) within an imaging spectrometer for wavelength encoding. This overcomes the intrinsic complexity and high cost of detection arrays with ps-resolving time capability. Unlike spatially offset Raman spectroscopy (SORS) or frequency offset Raman spectroscopy (FORS), TD-DIRS exploits the time-of-flight distribution of photons to probe the depth of the Raman signal at a single wavelength with a single source-detector separation. We validated the system using a bilayer tissue-bone mimicking phantom composed of a 1 cm thick slab of silicone overlaying a calcium carbonate specimen and demonstrated a high differentiation of the two Raman signals. We reconstructed the Raman spectra of the two layers, offering the potential for improved and quantitative material analysis. Using a bilayer phantom made of porcine muscle and calcium carbonate, we proved that our system can retrieve Raman peaks even in the presence of autofluorescence typical of biomedical tissues. Overall, our novel TD-DIRS setup proposes a cost-effective and high-performance approach for in-depth Raman spectroscopy in diffusive media. Competing Interests: The authors declare no potential conflicts of interest. (© 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.)
Databáze:	MEDLINE
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