Detection and absolute quantification of Lactiplantibacillus plantarum ATCC 202195 by quantitative real-time PCR.

Autor:	Shawon J; Nutrition and Clinical Services Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b) , Dhaka, Bangladesh., Pell LG; Centre for Global Child Health, The Hospital for Sick Children , Toronto, Ontario, Canada., Kabir M; Emerging Infections and Parasitology Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b) , Dhaka, Bangladesh., Evans K; International Flavors & Fragrances Inc. , Madison, Wisconsin, USA., Hasan M; Nutrition and Clinical Services Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b) , Dhaka, Bangladesh., Li G; Centre for Global Child Health, The Hospital for Sick Children , Toronto, Ontario, Canada., Qamar H; Centre for Global Child Health, The Hospital for Sick Children , Toronto, Ontario, Canada., Starke CWE; Centre for Global Child Health, The Hospital for Sick Children , Toronto, Ontario, Canada., Kurukulasuriya S; Centre for Global Child Health, The Hospital for Sick Children , Toronto, Ontario, Canada., Al Mahmud A; Nutrition and Clinical Services Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b) , Dhaka, Bangladesh., Sherman PM; Cell Biology Program, Research Institute, The Hospital for Sick Children , Toronto, Ontario, Canada.; Department of Paediatrics, Faculty of Medicine, University of Toronto , Toronto, Ontario, Canada., Sarker SA; Nutrition and Clinical Services Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b) , Dhaka, Bangladesh., Roth DE; Centre for Global Child Health, The Hospital for Sick Children , Toronto, Ontario, Canada.; Department of Paediatrics, Faculty of Medicine, University of Toronto , Toronto, Ontario, Canada., Haque R; Emerging Infections and Parasitology Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b) , Dhaka, Bangladesh.
Jazyk:	angličtina
Zdroj:	Microbiology spectrum [Microbiol Spectr] 2024 Jan 11; Vol. 12 (1), pp. e0271123. Date of Electronic Publication: 2023 Nov 29.
DOI:	10.1128/spectrum.02711-23
Abstrakt:	Importance: When administered for seven consecutive days shortly after birth, the probiotic bacterium Lactiplantibacillus plantarum ATCC 202195 plus fructooligosaccharide (FOS) was reported to reduce sepsis and lower respiratory tract infection events during early infancy in a randomized trial in India. Since probiotic effects are often strain specific, strain-level detection and quantification by routine molecular methods enables the monitoring of safety outcomes, such as probiotic-associated bacteremia, and allows for the quality of probiotic interventions to be assessed and monitored (i.e., verify strain identity and enumerate). Despite the potential clinical applications of L. plantarum ATCC 202195, an assay to detect and quantify this strain has not previously been described. Herein, we report the design of primer and probe sequences to detect L. plantarum ATCC 202195 and the development and optimization of a real-time PCR assay to detect and quantify the strain with high specificity and high sensitivity. Competing Interests: K.E. is an employee of International Flavors & Fragrances Inc., which manufactures L. plantarum ATCC 202195-containing probiotic/synbiotic preparations. D.E.R. received grant funding from the Bill & Melinda Gates Foundation to conduct a trial of L. plantarum ATCC 202195, for which International Flavors & Fragrances Inc. donated the probiotic and synbiotic used as investigational products.
Databáze:	MEDLINE
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