Targeted next-generation sequencing assay to detect 3 Moraxella spp. directly from bovine ocular swabs.

Autor:	Wilkes RP; Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA.; Department of Infectious Diseases (adjunct), University of Georgia, Athens, GA, USA., Kattoor JJ; Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA., Weng HY; Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA., Anis E; Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, New Bolton Center, PA, USA.
Jazyk:	angličtina
Zdroj:	Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc [J Vet Diagn Invest] 2024 Jan; Vol. 36 (1), pp. 120-123. Date of Electronic Publication: 2023 Nov 29.
DOI:	10.1177/10406387231216698
Abstrakt:	Infectious bovine keratoconjunctivitis (IBK) is associated with 2 species of Moraxella: M. bovis and M. bovoculi . A third novel Moraxella spp., designated tentatively as M. oculi , has been identified from the eyes of cattle with and without pinkeye. These 3 Moraxella spp. can be found in various combinations within the same clinical sample, making speciation of this genus directly from a sample impossible with Sanger sequencing. Assessing Moraxella diversity found in IBK- and non-IBK-affected cattle eyes, independent of culture, may provide additional information about IBK by avoiding the selectivity bias of culturing. We developed a targeted NGS panel to detect and speciate these 3 Moraxella spp. directly from bovine ocular swabs. Our targeted panel amplifies bacterial essential genes and the 16S-23S ribosomal RNA intergenic spacer region (ITS) of the 3 Moraxella spp. and speciates based on these sequences. Our panel was able to differentiate the 3 species directly from DNA extracted from 13 swabs (6 from healthy animals, 7 from animals with IBK), and every swab except one (clinically healthy eye) had the 3 Moraxella spp. Targeted NGS with sequencing of Moraxella spp. housekeeping genes appears to be a suitable method for speciation of Moraxella directly from ocular swabs. Competing Interests: Declaration of conflicting interestsThe authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Databáze:	MEDLINE
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