Anti-microbial efficacy of L-glutaminase (EC 3.5.1.2) against multidrug-resistant Pseudomonas aeruginosa infection.

Autor: Mahdi LH; Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq., Hasoon BA; Division of Biotechnology, Department of Applied Sciences, University of Technology, Baghdad, Iraq., Sulaiman GM; Division of Biotechnology, Department of Applied Sciences, University of Technology, Baghdad, Iraq. ghassan.m.sulaiman@uotechnology.edu.iq., Mohammed HA; Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Qassim University, Qassim, 51452, Saudi Arabia. ham.mohammed@qu.edu.sa.; Department of Pharmacognosy and Medicinal Plants, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt. ham.mohammed@qu.edu.sa., Jawad KH; Department of LASER and Optoelectronic Engineering, University of Technology, Baghdad, Iraq., Al-Dulimi AG; Department of Dentistry, Bilad Alrafidain University College, Diyala, 32001, Iraq., Essa RH; Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq., Albukhaty S; Department of Chemistry, College of Science, University of Misan, Maysan, 62001, Iraq.; College of Medicine, University of Warith Al-Anbiyaa, Karbala, Iraq., Khan R; Manav Rachna International Institute of Research and Study (MRIIRS), Faridabad, HR, 121 001, India. kahnriaz@gmail.com.
Jazyk: angličtina
Zdroj: The Journal of antibiotics [J Antibiot (Tokyo)] 2024 Feb; Vol. 77 (2), pp. 111-119. Date of Electronic Publication: 2023 Nov 28.
DOI: 10.1038/s41429-023-00678-z
Abstrakt: The aims of this study were isolation-purification and characterization of L-glutaminase from L. gasseri BRLHM clinical isolates and investigation of its efficiency as an antimicrobial agent against multidrug-resistant P. aeruginosa. The MICs of L-glutaminase and gentamicin reference were evaluated by the well-diffusion method. The biofilm on the IUD contraceptive was visualized using atomic force microscopy (AFM) image analyses. The purified L-glutaminase possessed significant antimicrobial activity against P. aeruginosa isolates (p < 0.05), and the antibiofilm formation activity of the purified L-glutaminase was stronger than the antibiofilm activity of the referral standard drug, gentamicin (P < 0.05), which were checked by the inhibition of the biofilm formation on the IUD contraceptive device. Investigations indicated that L-glutaminase may have a crucial role in future clinical applications.
(© 2023. The Author(s), under exclusive licence to the Japan Antibiotics Research Association.)
Databáze: MEDLINE
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