Interrogating ligand-receptor interactions using highly sensitive cellular biosensors.

Autor: Funk MA; Center for Pathophysiology, Infectiology and Immunology, Institute of Immunology, Division for Immune Receptors and T cell activation, Medical University of Vienna, Vienna, Austria., Leitner J; Center for Pathophysiology, Infectiology and Immunology, Institute of Immunology, Division for Immune Receptors and T cell activation, Medical University of Vienna, Vienna, Austria. judith.a.leitner@meduniwien.ac.at., Gerner MC; Division of Biomedical Science, University of Applied Sciences FH Campus Wien, Vienna, Austria., Hammerler JM; Division of Biomedical Science, University of Applied Sciences FH Campus Wien, Vienna, Austria., Salzer B; St. Anna Children's Cancer Research Institute, Vienna, Austria.; Christian Doppler Laboratory for Next Generation CAR T Cells, Vienna, Austria., Lehner M; St. Anna Children's Cancer Research Institute, Vienna, Austria.; Christian Doppler Laboratory for Next Generation CAR T Cells, Vienna, Austria., Battin C; Center for Pathophysiology, Infectiology and Immunology, Institute of Immunology, Division for Immune Receptors and T cell activation, Medical University of Vienna, Vienna, Austria., Gumpelmair S; Center for Pathophysiology, Infectiology and Immunology, Institute of Immunology, Division for Immune Receptors and T cell activation, Medical University of Vienna, Vienna, Austria., Stiasny K; Center for Virology, Medical University of Vienna, Vienna, Austria., Grabmeier-Pfistershammer K; Department of Dermatology, Medical University of Vienna, Vienna, Austria., Steinberger P; Center for Pathophysiology, Infectiology and Immunology, Institute of Immunology, Division for Immune Receptors and T cell activation, Medical University of Vienna, Vienna, Austria. peter.steinberger@meduniwien.ac.at.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Nov 28; Vol. 14 (1), pp. 7804. Date of Electronic Publication: 2023 Nov 28.
DOI: 10.1038/s41467-023-43589-1
Abstrakt: Interactions of membrane-resident proteins are important targets for therapeutic interventions but most methods to study them are either costly, laborious or fail to reflect the physiologic interaction of membrane resident proteins in trans. Here we describe highly sensitive cellular biosensors as a tool to study receptor-ligand pairs. They consist of fluorescent reporter cells that express chimeric receptors harboring ectodomains of cell surface molecules and intracellular signaling domains. We show that a broad range of molecules can be integrated into this platform and we demonstrate its applicability to highly relevant research areas, including the characterization of immune checkpoints and the probing of cells for the presence of receptors or ligands. The platform is suitable to evaluate the interactions of viral proteins with host receptors and to test for neutralization capability of drugs or biological samples. Our results indicate that cellular biosensors have broad utility as a tool to study protein-interactions.
(© 2023. The Author(s).)
Databáze: MEDLINE
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