Dual Nanoparticle Conjugates for Highly Sensitive and Versatile Sensing Using 19 F Magnetic Resonance Imaging.

Autor: Cooke DJ; Department of Chemistry, University of Texas at Austin, Austin, TX 78712, USA., Maier EY; College of Pharmacy, University of Texas at Austin, Austin, TX 78712, USA., King TL; Department of Chemistry, University of Texas at Austin, Austin, TX 78712, USA., Lin H; Department of Chemistry, University of Texas at Austin, Austin, TX 78712, USA., Hendrichs S; Department of Chemistry, University of Texas at Austin, Austin, TX 78712, USA., Lee S; Department of Chemistry, University of Texas at Austin, Austin, TX 78712, USA., Mafy NN; Department of Chemistry, University of Texas at Austin, Austin, TX 78712, USA., Scott KM; Department of Chemistry, University of Texas at Austin, Austin, TX 78712, USA., Lu Y; Department of Chemistry, University of Texas at Austin, Austin, TX 78712, USA.; Departments of chemical engineering, biomedical engineering, University of Texas at Austin, Austin, TX 78712, USA., Que EL; Department of Chemistry, University of Texas at Austin, Austin, TX 78712, USA.
Jazyk: angličtina
Zdroj: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2024 Jan 22; Vol. 63 (4), pp. e202312322. Date of Electronic Publication: 2023 Dec 20.
DOI: 10.1002/anie.202312322
Abstrakt: Fluorine magnetic resonance imaging ( 19 F MRI) has emerged as an attractive alternative to conventional 1 H MRI due to enhanced specificity deriving from negligible background signal in this modality. We report a dual nanoparticle conjugate (DNC) platform as an aptamer-based sensor for use in 19 F MRI. DNC consists of core-shell nanoparticles with a liquid perfluorocarbon core and a mesoporous silica shell ( 19 F-MSNs), which give a robust 19 F MR signal, and superparamagnetic iron oxide nanoparticles (SPIONs) as magnetic quenchers. Due to the strong magnetic quenching effects of SPIONs, this platform is uniquely sensitive and functions with a low concentration of SPIONs (4 equivalents) relative to 19 F-MSNs. The probe functions as a "turn-on" sensor using target-induced dissociation of DNA aptamers. The thrombin binding aptamer was incorporated as a proof-of-concept (DNC Thr ), and we demonstrate a significant increase in 19 F MR signal intensity when DNC Thr is incubated with human α-thrombin. This proof-of-concept probe is highly versatile and can be adapted to sense ATP and kanamycin as well. Importantly, DNC Thr generates a robust 19 F MRI "hot-spot" signal in response to thrombin in live mice, establishing this platform as a practical, versatile, and biologically relevant molecular imaging probe.
(© 2023 Wiley-VCH GmbH.)
Databáze: MEDLINE
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