Controlled Tempering of Lipid Concentration and Microbubble Shrinkage as a Possible Mechanism for Fine-Tuning Microbubble Size and Shell Properties.

Autor:	Zalloum IO; Department of Physics, Toronto Metropolitan University, Toronto M5B 2K3, Ontario, Canada.; Institute for Biomedical Engineering, Science and Technology (iBEST), A Partnership between Toronto Metropolitan University and St. Michael's Hospital, 209 Victoria Street, Toronto M5B 1T8, Ontario, Canada.; Keenan Research Centre for Biomedical Science, Unity Health Toronto, 209 Victoria Street, Toronto M5B 1W8, Ontario, Canada., Jafari Sojahrood A; Department of Physics, Toronto Metropolitan University, Toronto M5B 2K3, Ontario, Canada.; Institute for Biomedical Engineering, Science and Technology (iBEST), A Partnership between Toronto Metropolitan University and St. Michael's Hospital, 209 Victoria Street, Toronto M5B 1T8, Ontario, Canada.; Keenan Research Centre for Biomedical Science, Unity Health Toronto, 209 Victoria Street, Toronto M5B 1W8, Ontario, Canada., Paknahad AA; Department of Mechanical and Industrial Engineering, Toronto Metropolitan University, 350 Victoria Street, Toronto M5B 2K3, Ontario, Canada.; Institute for Biomedical Engineering, Science and Technology (iBEST), A Partnership between Toronto Metropolitan University and St. Michael's Hospital, 209 Victoria Street, Toronto M5B 1T8, Ontario, Canada.; Keenan Research Centre for Biomedical Science, Unity Health Toronto, 209 Victoria Street, Toronto M5B 1W8, Ontario, Canada., Kolios MC; Department of Physics, Toronto Metropolitan University, Toronto M5B 2K3, Ontario, Canada.; Institute for Biomedical Engineering, Science and Technology (iBEST), A Partnership between Toronto Metropolitan University and St. Michael's Hospital, 209 Victoria Street, Toronto M5B 1T8, Ontario, Canada.; Keenan Research Centre for Biomedical Science, Unity Health Toronto, 209 Victoria Street, Toronto M5B 1W8, Ontario, Canada., Tsai SSH; Department of Mechanical and Industrial Engineering, Toronto Metropolitan University, 350 Victoria Street, Toronto M5B 2K3, Ontario, Canada.; Institute for Biomedical Engineering, Science and Technology (iBEST), A Partnership between Toronto Metropolitan University and St. Michael's Hospital, 209 Victoria Street, Toronto M5B 1T8, Ontario, Canada.; Keenan Research Centre for Biomedical Science, Unity Health Toronto, 209 Victoria Street, Toronto M5B 1W8, Ontario, Canada.; Graduate Program in Biomedical Engineering, Toronto Metropolitan University, 350 Victoria Street, Toronto M5B 2K3, Ontario, Canada., Karshafian R; Department of Physics, Toronto Metropolitan University, Toronto M5B 2K3, Ontario, Canada.; Institute for Biomedical Engineering, Science and Technology (iBEST), A Partnership between Toronto Metropolitan University and St. Michael's Hospital, 209 Victoria Street, Toronto M5B 1T8, Ontario, Canada.; Keenan Research Centre for Biomedical Science, Unity Health Toronto, 209 Victoria Street, Toronto M5B 1W8, Ontario, Canada.
Jazyk:	angličtina
Zdroj:	Langmuir : the ACS journal of surfaces and colloids [Langmuir] 2023 Dec 12; Vol. 39 (49), pp. 17622-17631. Date of Electronic Publication: 2023 Nov 28.
DOI:	10.1021/acs.langmuir.3c01599
Abstrakt:	The acoustic response of microbubbles (MBs) depends on their resonance frequency, which is dependent on the MB size and shell properties. Monodisperse MBs with tunable shell properties are thus desirable for optimizing and controlling the MB behavior in acoustics applications. By utilizing a novel microfluidic method that uses lipid concentration to control MB shrinkage, we generated monodisperse MBs of four different initial diameters at three lipid concentrations (5.6, 10.0, and 16.0 mg/mL) in the aqueous phase. Following shrinkage, we measured the MB resonance frequency and determined its shell stiffness and viscosity. The study demonstrates that we can generate monodisperse MBs of specific sizes and tunable shell properties by controlling the MB initial diameter and aqueous phase lipid concentration. Our results indicate that the resonance frequency increases by 180-210% with increasing lipid concentration (from 5.6 to 16.0 mg/mL), while the bubble diameter is kept constant. Additionally, we find that the resonance frequency decreases by 260-300% with an increasing MB final diameter (from 5 to 12 μm), while the lipid concentration is held constant. For example, our results depict that the resonance frequency increases by ∼195% with increasing lipid concentration from 5.6 to 16.0 mg/mL, for ∼11 μm final diameter MBs. Additionally, we find that the resonance frequency decreases by ∼275% with increasing MB final diameter from 5 to 12 μm when we use a lipid concentration of 5.6 mg/mL. We also determine that MB shell viscosity and stiffness increase with increasing lipid concentration and MB final diameter, and the level of change depends on the degree of shrinkage experienced by the MB. Specifically, we find that by increasing the concentration of lipids from 5.6 to 16.0 mg/mL, the shell stiffness and viscosity of ∼11 μm final diameter MBs increase by ∼400 and ∼200%, respectively. This study demonstrates the feasibility of fine-tuning the MB acoustic response to ultrasound by tailoring the MB initial diameter and lipid concentration.
Databáze:	MEDLINE
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