Immunotoxicological, histopathological, and ultrastructural effects of waterborne pyrogallol exposure on African catfish (Clariasgariepinus).

Autor: Hamed M; Department of Zoology, Faculty of Science, Al-Azhar University (Assiut Branch), Assiut, 71524, Egypt. Electronic address: mohamedhamedsayed239@gmail.com., Said REM; Department of Zoology, Faculty of Science, Al-Azhar University (Assiut Branch), Assiut, 71524, Egypt., Soliman HAM; Department of Zoology, Faculty of Science, Sohag University, Sohag 8562, Egypt., Osman AGM; Department of Zoology, Faculty of Science, Al-Azhar University (Assiut Branch), Assiut, 71524, Egypt., Martyniuk CJ; Center for Environmental and Human Toxicology, Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USA.
Jazyk: angličtina
Zdroj: Chemosphere [Chemosphere] 2024 Feb; Vol. 349, pp. 140792. Date of Electronic Publication: 2023 Nov 26.
DOI: 10.1016/j.chemosphere.2023.140792
Abstrakt: Pyrogallol is a naturally occurring polyphenol derived from natural plants, such as Acer rubrum and Eucalyptus sp. The current study was designed to evaluated pyrogallol-mediated toxicity at sublethal levels (1, 5, and 10 mg/L), derived from 96 h-LC 50 values previously determined for African catfish (Clarias gariepinus). Immunotoxicological indices, histological, histochemical, and ultrastructural alterations in C. gariepinus were evaluated following a 15-day pyrogallol exposure. Pyrogallol decreased immune parameters [lysozyme activity (LYZ), immunoglobulin M (IgM), and phagocytic activity] and increased pro-inflammatory cytokines, interleukin-1 beta (IL-1β), interleukin-6 (IL-6) in the serum of C. gariepinus. In addition, histopathology analysis demonstrated that exposure to pyrogallol induced injury in the liver and spleen of fish. Cellular changes in the liver include hepatocyte hydropic degeneration, melanomacrophage, vacuolated hepatocytes, congested blood, severe structural deformation, and hemorrhage. In the spleen, ellipsoid structures, melanomacrophage centers, and infiltration of inflammatory cells were evident. Together, a high frequency of histopathological lesions was scored in both the liver and spleen of C. gariepinus, which showed a dose-dependent relationship between pyrogallol exposure and histopathological indices. Our data suggest that dysfunction in the immune system may be mediated by pyrogallol-induced changes in cytokines.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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