| Autor: |
A Hama H; Department of Biology, Faculty of Education, Tishk International University, Erbil, Kurdistan Region of Iraq. harmand.ali@tiu.edu.iq., Hasan BS; Awat Radiation Oncology Center, Erbil, Kurdistan Region, Iraq. dr.bestoon@hotmail.com., Barzngy B; Oncology Center, Rizgary Hospital, Erbil, Kurdistan Region, Iraq. barzngybasak@gmail.com., Abdulla SS; Department of Oral Diagnosis and Oral Medicine, College of Dentistry, Hawler Medical University, Erbil, Kurdistan Region, Iraq. samhisto@gmail.com., Karim AY; Department of Biology, College of Science, Salahaddin University-Erbil, Kurdistan region, Iraq. rozhgar.mohammed@su.edu.krd., Khailany RA; Department of Biology, College of Science, Salahaddin University-Erbil, Kurdistan region, Iraq. abdulkarim.karim@su.edu.krd., Miasko M; Department of Basic Science, Faculty of Dentistry, Tishk International University, Erbil, Kurdistan Region, Iraq. monika.miasko@tiu.edu.iq., Dabrowski JM; Faculty of Chemistry, Jagiellonian University, Krakow, Poland. jdabrows@chemia.uj.edu.pl., Pucelik B; Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland. barbara.pucelik@uj.edu.pl. |
| Abstrakt: |
Colorectal cancer (CRC) is the third leading cause of cancer-related deaths worldwide. The tumor suppressor gene MT-CO1, and Kristen Rat Sarcoma Virus (KRAS), an oncogene are primarily responsible for controlling cell apoptosis, cell cycle arrest, and cell proliferation, and any irregularities in these genes could lead to cancer. This study aims to examine the expression of KRAS and MT-CO1 in CRC biopsy specimens and investigate their relationship with one another in CRC patients residing in the Erbil city of Kurdistan Region, Iraq. The study involved categorizing 42 sets of colorectal cancer tissues and their corresponding controls based on their types and patients' clinical characteristics. The expression of KRAS and MT-CO1 in the samples was assessed using Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR), with statistical significance set at p<0.05. The expression of KRAS was found to be significantly higher in CRC compared to the control (n=42, p=0.0001). On the other hand, the expression of MT-CO1 did not exhibit significant differences compared to the control group with a p-value of 0.12. Furthermore, the Chi-square and correlation analysis results depicted that MT-CO1 expression negatively correlates with KRAS expression (p= 0.0001, r= -0.047) in CRC tissues. In conclusion, the variation in the expression of KRAS and MT-CO1, and their correlations could potentially serve as a good indicator in the detection and prognosis of CRC, which might lead to better translational research on the same. However, for a better understanding of the underlying mechanisms, further analysis is required. |
