| Autor: |
Postec A; Aix Marseille Univ, Université de Toulon, CNRS, IRD, MIO, Marseille, France., Galès G; Aix Marseille Univ, Université de Toulon, CNRS, IRD, MIO, Marseille, France., Prime AH; Aix Marseille Univ, Université de Toulon, CNRS, IRD, MIO, Marseille, France., Bartoli M; Aix Marseille Univ, Université de Toulon, CNRS, IRD, MIO, Marseille, France., Price RE; Stony Brook University, SoMAS Stony Brook, New York 11794, USA., Vandecasteele C; INRAE, US 1426, GeT-PlaGe, Genotoul, France Genomique, Université Fédérale de Toulouse, Castanet-Tolosan, France., Erauso G; Aix Marseille Univ, Université de Toulon, CNRS, IRD, MIO, Marseille, France. |
| Abstrakt: |
A novel thermophilic strain, designated BP5-C20A T , was isolated from the shallow hydrothermal field of the Panarea island in the Aeolian archipelago close to Sicily, Italy. Cells are motile rods surrounded with a 'toga', Gram-stain-negative and display a straight to curved morphology during the exponential phase. Strain BP5-C20A T is thermophilic (optimum 55 °C), moderately acidophilic (optimum pH 5.6) and halotolerant (optimum 25 g l -1 NaCl). It can use yeast extract, peptone and tryptone. It uses the following carbohydrates: cellobiose, fructose, glucose, maltose, starch, sucrose and xylan. Elemental sulphur is used as an electron acceptor and reduced to hydrogen sulphide. The predominant cellular fatty acid is C 16 : 0 . Phylogenetic analysis showed that strain BP5-C20A T shared 97.3 % 16S rRNA gene sequence identity with the closest related species Marinitoga lauensis LG1 T . The complete genome of strain BP5-C20A T is 2.44 Mb in size with a G+C content of 27.3 mol%. The dDDH and ANI values between the genomes of strains BP5-C20A T and M. lauensis LG1 T are 31.0 and 85.70% respectively. Finally, from its physiological, metabolic and genomic characteristics, strain BP5-C20A T (=DSM 112332 T =JCM 39183 T ) is proposed as representative of a novel species of the genus Marinitoga named Marinitoga aeolica sp. nov. and belonging to the order Petrotogales , in the phylum Thermotogota . |
