Robust organ size in Arabidopsis is primarily governed by cell growth rather than cell division patterns.

Autor:	Burda I; Genetics, Genomics, and Development Graduate Program, Cornell University, Ithaca, NY 14850, USA.; Weill Institute for Cell and Molecular Biology Cornell University, Ithaca, NY, 14850, USA.; School of Integrative Plant Science, Section of Plant Biology, Cornell University, Ithaca, NY, 14850, USA., Li CB; Department of Mathematics, Stockholm University, Stockholm 10691, Sweden., Clark FK; Genetics, Genomics, and Development Graduate Program, Cornell University, Ithaca, NY 14850, USA.; Weill Institute for Cell and Molecular Biology Cornell University, Ithaca, NY, 14850, USA.; School of Integrative Plant Science, Section of Plant Biology, Cornell University, Ithaca, NY, 14850, USA., Roeder AHK; Genetics, Genomics, and Development Graduate Program, Cornell University, Ithaca, NY 14850, USA.; Weill Institute for Cell and Molecular Biology Cornell University, Ithaca, NY, 14850, USA.; School of Integrative Plant Science, Section of Plant Biology, Cornell University, Ithaca, NY, 14850, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2023 Nov 20. Date of Electronic Publication: 2023 Nov 20.
DOI:	10.1101/2023.11.11.566685
Abstrakt:	Organ sizes and shapes are highly reproducible, or robust, within a species and individuals. Arabidopsis thaliana sepals, which are the leaf-like organs that enclose flower buds, have consistent size and shape, which indicates robust development. Counterintuitively, variability in cell growth rate over time and between cells facilitates robust development because cumulative cell growth averages to a uniform rate. Here we investigate how sepal morphogenesis is robust to changes in cell division but not robust to changes in cell growth variability. We live image and quantitatively compare the development of sepals with increased or decreased cell division rate ( lgo mutant and LGO overexpression, respectively), a mutant with altered cell growth variability ( ftsh4 ), and double mutants combining these. We find that robustness is preserved when cell division rate changes because there is no change in the spatial pattern of growth. Meanwhile when robustness is lost in ftsh4 mutants, cell growth accumulates unevenly, and cells have disorganized growth directions. Thus, we demonstrate in vivo that both cell growth rate and direction average in robust development, preserving robustness despite changes in cell division. Competing Interests: Competing interests The authors declare they have no competing interests.
Databáze:	MEDLINE
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