| Autor: |
Schmidt AK; Division of Biological Sciences, University of Montana, Missoula, Montana, USA., Schwartzkopf CM; Division of Biological Sciences, University of Montana, Missoula, Montana, USA., Pourtois JD; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA., Burgener E; Department of Pediatrics, Division of Pulmonology, Children's Hospital of Los Angeles, University of Southern California, Los Angeles, CA, USA., Faith DR; Division of Biological Sciences, University of Montana, Missoula, Montana, USA., Joyce A; Division of Biological Sciences, University of Montana, Missoula, Montana, USA., Lamma T; Division of Biological Sciences, University of Montana, Missoula, Montana, USA., Kumar G; School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Kerala, India., Bollyky PL; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA., Secor PR; Division of Biological Sciences, University of Montana, Missoula, Montana, USA. |
| Abstrakt: |
Pseudomonas aeruginosa is an opportunistic bacterial pathogen that commonly causes medical hardware, wound, and respiratory infections. Temperate filamentous Pf phages that infect P. aeruginosa impact numerous bacterial virulence phenotypes. Most work on Pf phages has focused on strain Pf4 and its host P. aeruginosa PAO1. Expanding from Pf4 and PAO1, this study explores diverse Pf strains infecting P. aeruginosa clinical isolates. We describe a simple technique targeting the Pf lysogeny maintenance gene, pflM ( PA0718 ), that enables the effective elimination of Pf prophages from diverse P. aeruginosa hosts. This study also assesses the effects different Pf phages have on host quorum sensing, biofilm formation, virulence factor production, and virulence. Collectively, this research not only introduces a valuable tool for Pf prophage elimination from diverse P. aeruginosa isolates, but also advances our understanding of the complex relationship between P. aeruginosa and filamentous Pf phages. |
