Time- and distance-resolved robotic imaging of fluid flow in vertical microfluidic strips: a new technique for quantitative, multiparameter measurement of global haemostasis.

Autor:	Sarıyer RM; Reading School of Pharmacy, University of Reading Whiteknights Reading RG6 6UB UK r.sariyer@pgr.reading.ac.uk kkgill@uvic.ca s.h.needs@reading.ac.uk a.d.edwards@reading.ac.uk +44 (0)118 378 4253., Gill K; Reading School of Pharmacy, University of Reading Whiteknights Reading RG6 6UB UK r.sariyer@pgr.reading.ac.uk kkgill@uvic.ca s.h.needs@reading.ac.uk a.d.edwards@reading.ac.uk +44 (0)118 378 4253.; Department of Chemical Engineering and Centre for Biosensors, Bioelectronics and Biodevices (CBio), University of Bath Bath BA2 7AY UK n.m.reis@bath.ac.uk., Needs SH; Reading School of Pharmacy, University of Reading Whiteknights Reading RG6 6UB UK r.sariyer@pgr.reading.ac.uk kkgill@uvic.ca s.h.needs@reading.ac.uk a.d.edwards@reading.ac.uk +44 (0)118 378 4253., Hodge D; Reading School of Biological Sciences, University of Reading Whiteknights Reading UK d.j.hodge@pgr.reading.ac.uk c.i.jones@reading.ac.uk., Reis NM; Department of Chemical Engineering and Centre for Biosensors, Bioelectronics and Biodevices (CBio), University of Bath Bath BA2 7AY UK n.m.reis@bath.ac.uk., Jones CI; Reading School of Biological Sciences, University of Reading Whiteknights Reading UK d.j.hodge@pgr.reading.ac.uk c.i.jones@reading.ac.uk., Edwards AD; Reading School of Pharmacy, University of Reading Whiteknights Reading RG6 6UB UK r.sariyer@pgr.reading.ac.uk kkgill@uvic.ca s.h.needs@reading.ac.uk a.d.edwards@reading.ac.uk +44 (0)118 378 4253.; School of Electronics and Computer Science, University of Southampton Highfield Southampton SO17 1BJ UK.
Jazyk:	angličtina
Zdroj:	Sensors & diagnostics [Sens Diagn] 2023 Oct 17; Vol. 2 (6), pp. 1623-1637. Date of Electronic Publication: 2023 Oct 17 (Print Publication: 2023).
DOI:	10.1039/d3sd00162h
Abstrakt:	Measuring the complex processes of blood coagulation, haemostasis and thrombosis that are central to cardiovascular health and disease typically requires a choice between high-resolution low-throughput laboratory assays, or simpler less quantitative tests. We propose combining mass-produced microfluidic devices with open-source robotic instrumentation to enable rapid development of affordable and portable, yet high-throughput and performance haematological testing. A time- and distance-resolved fluid flow analysis by Raspberry Pi imaging integrated with controlled sample addition and illumination, enabled simultaneous tracking of capillary rise in 120 individual capillaries (∼160, 200 or 270 μm internal diameter), in 12 parallel disposable devices. We found time-resolved tracking of capillary rise in each individual microcapillary provides quantitative information about fluid properties and most importantly enables quantitation of dynamic changes in these properties following stimulation. Fluid properties were derived from flow kinetics using a pressure balance model validated with glycerol-water mixtures and blood components. Time-resolved imaging revealed fluid properties that were harder to determine from a single endpoint image or equilibrium analysis alone. Surprisingly, instantaneous superficial fluid velocity during capillary rise was found to be largely independent of capillary diameter at initial time points. We tested if blood function could be measured dynamically by stimulating blood with thrombin to trigger activation of global haemostasis. Thrombin stimulation slowed vertical fluid velocity consistent with a dynamic increase in viscosity. The dynamics were concentration-dependent, with highest doses reducing flow velocity faster (within 10 s) than lower doses (10-30 s). This open-source imaging instrumentation expands the capability of affordable microfluidic devices for haematological testing, towards high-throughput multi-parameter blood analysis needed to understand and improve cardiovascular health. Competing Interests: A. D. Edwards and N. M. Reis are the inventors of patent application protecting aspects of the novel microfluidic devices tested in this study and is a director and shareholder in Capillary Film Technology Ltd, a company holding a commercial license to this patent application: WO2016012778 “Capillary assay device with internal hydrophilic coating” inventors AD Edwards, NM Reis. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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