Leishmanicidal and healing effects of 3β,6β,16β-trihydroxy lup-20 (29)-ene isolated from Combretum leprosum on Leishmania braziliensis infection in vitro and in vivo.

Autor: Sombra Santos A; Parasitology Laboratory, Department of Pathology and Legal Medicine, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil., de Castro Rodrigues NL; Parasitology Laboratory, Department of Pathology and Legal Medicine, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil., Marciano Fonseca FR; Parasitology Laboratory, Department of Pathology and Legal Medicine, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.; Laboratory of Pharmacognosy and Pharmaceutical Technology, Department of Pharmacy, Faculty of Pharmacy Dentistry and Nursing, Federal University of Ceará, Fortaleza, Ceará, Brazil., Braga Fayão Oliveira N; Parasitology Laboratory, Department of Pathology and Legal Medicine, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil., Oliveira Loucard B; Laboratory of Pharmacognosy and Pharmaceutical Technology, Department of Pharmacy, Faculty of Pharmacy Dentistry and Nursing, Federal University of Ceará, Fortaleza, Ceará, Brazil., Fernandes Heredia F; Parasitology Laboratory, Department of Pathology and Legal Medicine, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil., Albuquerque Gomes Nogueira TN; Parasitology Laboratory, Department of Pathology and Legal Medicine, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil., Monteiro Abreu T; Parasitology Laboratory, Department of Pathology and Legal Medicine, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil., Silva Dos Santos H; Universidade Estadual Vale do Acaraú, Sobral, Ceará, Brazil., Holanda Teixeira E; Integrated Laboratory of Biomolecules, Department of Pathology and Legal Medicine, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil., Almeida Moreira Leal LK; Laboratory of Pharmacognosy and Pharmaceutical Technology, Department of Pharmacy, Faculty of Pharmacy Dentistry and Nursing, Federal University of Ceará, Fortaleza, Ceará, Brazil., Brandim Gomes RB; FIOCRUZ Ceará, Oswaldo Cruz Foundation, Eusébio, Fortaleza, Ceará, Brazil., Romero Teixeira C; FIOCRUZ Ceará, Oswaldo Cruz Foundation, Eusébio, Fortaleza, Ceará, Brazil., Teixeira MJ; Parasitology Laboratory, Department of Pathology and Legal Medicine, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2023 Nov 27; Vol. 18 (11), pp. e0287665. Date of Electronic Publication: 2023 Nov 27 (Print Publication: 2023).
DOI: 10.1371/journal.pone.0287665
Abstrakt: Treatment of cutaneous leishmaniasis depends on drugs that potentially cause serious side effects and resistance. Thus, topical therapies are attractive alternatives to the drugs currently used. 3β, 6β, 16β-trihydroxylup-20 (29)-ene is a lupane triterpene isolated from Combretum leprosum Mart. leaves (CLF-1), with reports of in vitro antileishmanial effect against L. amazonensis and to promote lesion healing in animal model. Herein, we evaluated the in vitro and in vivo antileishmanial and healing effects of CLF-1 against L. braziliensis. CLF-1 treatment showed low toxicity in macrophages and significantly reduced parasite load in vitro. CLF-1 induced higher IL-12 and TNF-α production and more discrete IL-4 and IL-10 production. For in vivo evaluation, a CLF-1 cream formulation was prepared to treat hamsters infected with L. braziliensis. CLF-1 treatment was able to reduce parasite load of the infected skin and lymph node more efficiently than the conventional treatment. Histopathological analysis indicated a strong inflammatory response accompanied by an important healing response. Data from this study indicate that topical CLF-1 treatment was effective and non-toxic in L. braziliensis infected hamsters suggesting its potential for further development as a future therapeutic intervention.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Sombra Santos et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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