Characterization of HcaA, a novel autotransporter protein in Helicobacter cinaedi , and its role in host cell adhesion.

Autor:	Aoki S; Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan., Mori S; Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan., Matsui H; Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan., Shibayama K; Department of Bacteriology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan., Kenri T; Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan., Rimbara E; Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.
Jazyk:	angličtina
Zdroj:	MSphere [mSphere] 2023 Dec 20; Vol. 8 (6), pp. e0040323. Date of Electronic Publication: 2023 Nov 27.
DOI:	10.1128/msphere.00403-23
Abstrakt:	Importance: Helicobacter species are classified as gastric or enterohepatic according to their habitat. Among enterohepatic Helicobacter species, which inhabit the intestine, colon, and liver, Helicobacter cinaedi has been most frequently isolated from humans. H. cinaedi often causes bacteremia and cellulitis in immunocompromised hosts. Here, we focused on the H. cinaedi autotransporter protein A (HcaA), a novel virulence factor in H. cinaedi . We discovered that HcaA contributes to cell adhesion via its Arg-Gly-Asp motif. Furthermore, in animal experiments, bacterial colonization was reduced in mice infected with HcaA-knockout strains, supporting the hypothesis that HcaA contributes to H. cinaedi adhesion to host cells. Our study provides a novel mechanism for the establishment of H. cinaedi infections and provides new insights into the role of autotransporter proteins in the establishment of Helicobacter infection. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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