Comparative pathogenomic analysis reveals a highly tetanus toxin-producing clade of Clostridium tetani isolates in Japan.

Autor:	Shitada C; Toxin and Biologicals Research Laboratory, Kumamoto Health Science University, Kumamoto, Japan.; The Chemo-Sero-Therapeutic Research Institute, Kumamoto, Japan., Sekizuka T; Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo, Japan., Yamamoto A; Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan., Sakamoto C; Toxin and Biologicals Research Laboratory, Kumamoto Health Science University, Kumamoto, Japan.; The Chemo-Sero-Therapeutic Research Institute, Kumamoto, Japan., Hashino M; Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo, Japan., Kuroda M; Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo, Japan., Takahashi M; Toxin and Biologicals Research Laboratory, Kumamoto Health Science University, Kumamoto, Japan.; The Chemo-Sero-Therapeutic Research Institute, Kumamoto, Japan.
Jazyk:	angličtina
Zdroj:	MSphere [mSphere] 2023 Dec 20; Vol. 8 (6), pp. e0036923. Date of Electronic Publication: 2023 Nov 27.
DOI:	10.1128/msphere.00369-23
Abstrakt:	Importance: C. tetani is a spore-forming, anaerobic bacterium that produces a toxin causing muscle stiffness and paralysis. Tetanus is preventable with the toxoid vaccine, but it remains a significant public health threat in regions with low vaccine coverage. However, there are relatively few isolates and limited genomic information available worldwide. In Japan, about 100 cases are reported each year, but there have been no nationwide surveys of isolates, and no genomic information from Japanese isolates has been published. In our study, we analyzed the genomes of 151 strains from a limited survey of soil in Kumamoto, Japan. Our findings revealed a high degree of genetic diversity, and we also identified a subset of strains that produced significantly more toxin, which provides new insights into the pathogenesis of tetanus. Our findings lay the foundation for future studies to investigate the distribution and evolution of C. tetani in Japan and neighboring countries. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Motohide Takahashi has received an advisory contract from The Chemo-Sero-Therapeutic Research Institute.
Databáze:	MEDLINE
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