Improving biosensor accuracy and speed using dynamic signal change and theory-guided deep learning.

Autor: Zhang J; Grado Department of Industrial and Systems Engineering, Virginia Tech, Blacksburg, VA, 24061, USA., Srivatsa P; Department of Computer Science, Virginia Tech, Blacksburg, VA, 24061, USA., Ahmadzai FH; Grado Department of Industrial and Systems Engineering, Virginia Tech, Blacksburg, VA, 24061, USA., Liu Y; Grado Department of Industrial and Systems Engineering, Virginia Tech, Blacksburg, VA, 24061, USA; School of Neuroscience, Virginia Tech, Blacksburg, VA, 24061, USA., Song X; Grado Department of Industrial and Systems Engineering, Virginia Tech, Blacksburg, VA, 24061, USA., Karpatne A; Department of Computer Science, Virginia Tech, Blacksburg, VA, 24061, USA., Kong ZJ; Grado Department of Industrial and Systems Engineering, Virginia Tech, Blacksburg, VA, 24061, USA., Johnson BN; Grado Department of Industrial and Systems Engineering, Virginia Tech, Blacksburg, VA, 24061, USA; School of Neuroscience, Virginia Tech, Blacksburg, VA, 24061, USA; Department of Materials Science and Engineering, Virginia Tech, Blacksburg, VA, 24061, USA; Department of Chemical Engineering, Virginia Tech, Blacksburg, VA, 24061, USA. Electronic address: bnj@vt.edu.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2024 Feb 15; Vol. 246, pp. 115829. Date of Electronic Publication: 2023 Nov 14.
DOI: 10.1016/j.bios.2023.115829
Abstrakt: False results and time delay are longstanding challenges in biosensing. While classification models and deep learning may provide new opportunities for improving biosensor performance, such as measurement confidence and speed, it remains a challenge to ensure that predictions are explainable and consistent with domain knowledge. Here, we show that consistency of deep learning classification model predictions with domain knowledge in biosensing can be achieved by cost function supervision and enables rapid and accurate biosensing using the biosensor dynamic response. The impact and utility of the methodology were validated by rapid and accurate quantification of microRNA (let-7a) across the nanomolar (nM) to femtomolar (fM) concentration range using the dynamic response of cantilever biosensors. Data augmentation and cost function supervision based on the consistency of model predictions and experimental observations with the theory of surface-based biosensors improved the F1 score, precision, and recall of a recurrent neural network (RNN) classifier by an average of 13.8%. The theory-guided RNN (TGRNN) classifier enabled quantification of target analyte concentration and false results with an average prediction accuracy, precision, and recall of 98.5% using the initial transient or entire dynamic response, which is indicative of high prediction accuracy and low probability of false-negative and false-positive results. Classification scores were used to establish new relationships among biosensor performance characteristics (e.g., measurement confidence) and design parameters (e.g., inputs and hyperparameters of classification models and data acquisition parameters) that may be used for characterizing biosensor performance.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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