A novel device for the determination of liposome/water partition coefficients.

Autor: Jerga R; Department of Analytical Chemistry, Faculty of Science, Palacký University, 17. listopadu 1192/12, 771 46, Olomouc, Czech Republic., Brablecová V; Department of Analytical Chemistry, Faculty of Science, Palacký University, 17. listopadu 1192/12, 771 46, Olomouc, Czech Republic., Talášková V; Department of Analytical Chemistry, Faculty of Science, Palacký University, 17. listopadu 1192/12, 771 46, Olomouc, Czech Republic., Tomková H; Department of Analytical Chemistry, Faculty of Science, Palacký University, 17. listopadu 1192/12, 771 46, Olomouc, Czech Republic., Součková J; Department of Analytical Chemistry, Faculty of Science, Palacký University, 17. listopadu 1192/12, 771 46, Olomouc, Czech Republic., Barták P; Department of Analytical Chemistry, Faculty of Science, Palacký University, 17. listopadu 1192/12, 771 46, Olomouc, Czech Republic., Skopalová J; Department of Analytical Chemistry, Faculty of Science, Palacký University, 17. listopadu 1192/12, 771 46, Olomouc, Czech Republic. Electronic address: jana.skopalova@upol.cz.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2024 Mar 01; Vol. 269, pp. 125434. Date of Electronic Publication: 2023 Nov 19.
DOI: 10.1016/j.talanta.2023.125434
Abstrakt: A novel, cheap and easy-to-construct device and a simple method for partition coefficient determination in liposome/water system based on modified equilibrium dialysis have been developed. The device consists of two vials separated by a semi-permeable membrane, through which the free form of a low molecular weight substance is transported by shaking assisted diffusion. Five test substances, eugenol, carvacrol, thymol, 4-hydroxybenzyl alcohol (4-HBA) and butylparaben were analyzed after equilibration in aqueous phase by three methods, HPLC-UV, GC-MS and DPV with comparable results. This shows the possibility of using the proposed method in any laboratory with any equipment capable of analyzing the substance under study. The liposome/water partition coefficients (log P l/w ) determined for eugenol (2.39), thymol (2.83), carvacrol (2.78) and butylparaben (3.30) are consistent with previously published data. A strong effect of NaCl on the liposome/water partition coefficient was observed. The value of log P l/w  = 1.06 determined for 4-HBA in the presence of 0.15 mol L -1 NaCl in the partitioning liposomal system was considerably lower than in the absence of the salt (log P l/w  = 2.06). The developed method was used to determine the partition coefficient of morphine in liposome/water system without NaCl (log P l/w  = 2.65) under given conditions.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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